Gögele Clemens, Müller Silvana, Belov Svetlana, Pradel Andreas, Wiltzsch Sven, Lenhart Armin, Hornfeck Markus, Kerling Vera, Rübling Achim, Kühl Hannes, Schäfer-Eckart Kerstin, Minnich Bernd, Weiger Thomas Martin, Schulze-Tanzil Gundula
Institute of Anatomy and Cell Biology, Paracelsus Medical University, Nuremberg and Salzburg, 90419 Nuremberg, Germany.
Department of Biosciences and Medical Biology, Paris Lodron University Salzburg, 5020 Salzburg, Austria.
Cells. 2022 May 7;11(9):1577. doi: 10.3390/cells11091577.
Regeneration of articular cartilage remains challenging. The aim of this study was to increase the stability of pure bioactive glass (BG) scaffolds by means of solvent phase polymer infiltration and to maintain cell adherence on the glass struts. Therefore, BG scaffolds either pure or enhanced with three different amounts of poly(D-L-lactide-co-glycolide) (PLGA) were characterized in detail. Scaffolds were seeded with primary porcine articular chondrocytes (pACs) and human mesenchymal stem cells (hMSCs) in a dynamic long-term culture (35 days). Light microscopy evaluations showed that PLGA was detectable in every region of the scaffold. Porosity was greater than 70%. The biomechanical stability was increased by polymer infiltration. PLGA infiltration did not result in a decrease in viability of both cell types, but increased DNA and sulfated glycosaminoglycan (sGAG) contents of hMSCs-colonized scaffolds. Successful chondrogenesis of hMSC-colonized scaffolds was demonstrated by immunocytochemical staining of collagen type II, cartilage proteoglycans and the transcription factor SOX9. PLGA-infiltrated scaffolds showed a higher relative expression of cartilage related genes not only of pAC-, but also of hMSC-colonized scaffolds in comparison to the pure BG. Based on the novel data, our recommendation is BG scaffolds with single infiltrated PLGA for cartilage tissue engineering.
关节软骨的再生仍然具有挑战性。本研究的目的是通过溶剂相聚合物浸润来提高纯生物活性玻璃（BG）支架的稳定性，并保持细胞在玻璃支柱上的黏附。因此，对纯BG支架以及用三种不同量的聚（D - L - 丙交酯 - 共 - 乙交酯）（PLGA）增强的BG支架进行了详细表征。在动态长期培养（35天）中，将原代猪关节软骨细胞（pACs）和人间充质干细胞（hMSCs）接种到支架上。光学显微镜评估显示，在支架的每个区域都可检测到PLGA。孔隙率大于70%。聚合物浸润提高了生物力学稳定性。PLGA浸润并未导致两种细胞类型的活力下降，但增加了hMSCs定植支架的DNA和硫酸化糖胺聚糖（sGAG）含量。通过对II型胶原蛋白、软骨蛋白聚糖和转录因子SOX9进行免疫细胞化学染色，证明了hMSC定植支架成功发生软骨形成。与纯BG相比，PLGA浸润的支架不仅在pAC定植的支架中，而且在hMSC定植的支架中都显示出更高的软骨相关基因相对表达。基于这些新数据，我们推荐用于软骨组织工程的单浸润PLGA的BG支架。
