Wang Wenhui, He Jun, Wang Yunlong, Li Na, Sun Quanguang, Zhang Menglong, Li Qi, Xu Haosen, Shao Kun, Zhang Shihao, Liu Shijia, Chen Liangming, Liu Xi, Tian Yunlu, Zhao Zhigang, Liu Yuqiang, Wan Jianmin
State Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Jiangsu Engineering Research Center for Plant Genome Editing, National Observation and Research Station of Rice Germplasm Resources, Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, Nanjing, China.
Biological Breeding Zhongshan Laboratory, Nanjing, China.
Theor Appl Genet. 2025 Aug 6;138(8):199. doi: 10.1007/s00122-025-04978-w.
The brown planthopper (BPH) poses a persistent threat to global rice production as one of the most devastating insect pests. Development and utilization of BPH-resistant rice cultivars are believed to be the most sustainable and eco-friendly control strategy, which has been successfully implemented in indica rice subspecies but remains underdeveloped in japonica rice. Here, we introgressed the BPH resistance gene Bph27(t) into elite japonica cultivars. Although the introgressed lines demonstrated robust BPH resistance throughout all developmental stages, they unexpectedly exhibited a brown anther phenotype accompanied by significantly reduced spikelet fertility. Histological analyses revealed that the brown anther phenotype and spikelet sterility in Bph27(t)-carrying japonica plants were likely caused by delayed tapetum degradation, leading to anther indehiscence. Furthermore, a brown anther locus, Ba1, was identified and localized to a 1.3 Mb region closely linked to Bph27(t). Genome assembly of the donor parent, Balamawee, revealed an approximately 1.1 Mb inversion in the fine-mapped region. By breaking the genetic linkage between Bph27(t) and Ba1, BPH-resistant japonica rice with high seed-setting rates was subsequently developed. The identification of Ba1 provides a new genetic resource to further explore the molecular mechanisms underlying rice anther development. Moreover, the development of novel BPH-resistant japonica germplasm lays the foundation for breeding high-yield and BPH-resistant japonica rice varieties.
褐飞虱作为最具破坏性的害虫之一，对全球水稻生产构成持续威胁。培育和利用抗褐飞虱水稻品种被认为是最可持续且生态友好的防治策略，这一策略已在籼稻亚种中成功实施，但在粳稻中仍未得到充分发展。在此，我们将抗褐飞虱基因Bph27(t)渗入优良粳稻品种。尽管渗入系在所有发育阶段均表现出强大的抗褐飞虱能力，但它们意外地表现出花药褐色的表型，同时小穗育性显著降低。组织学分析表明，携带Bph27(t)的粳稻植株中花药褐色表型和小穗不育可能是由于绒毡层降解延迟导致花药不开裂所致。此外，还鉴定出一个花药褐色位点Ba1，并将其定位到与Bph27(t)紧密连锁的1.3 Mb区域。供体亲本Balamawee的基因组组装显示，在精细定位区域存在一个约1.1 Mb的倒位。通过打破Bph27(t)与Ba1之间的遗传连锁，随后培育出了结实率高的抗褐飞虱粳稻。Ba1的鉴定为进一步探索水稻花药发育的分子机制提供了新的遗传资源。此外，新型抗褐飞虱粳稻种质的培育为高产抗褐飞虱粳稻品种的选育奠定了基础。
