Daneshyar Fahimeh, Alikhani Mohammad Yousef, Tayebi Soudeh, Abedi Firouzjaei Delaram
Department of Pediatric dentistry, Faculty of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran.
Infectious Disease Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran.
Int J Mol Cell Med. 2025 Jul 1;14(2):637-645. doi: 10.22088/IJMCM.BUMS.14.2.637. eCollection 2025.
Dental caries is among the most prevalent chronic diseases. It arises from bacterial biofilm formation on tooth surfaces due to metabolic activity. (. ) is a key pathogen implicated in the development of dental caries. As bacterial resistance to conventional treatments increases, there is a growing interest in using novel compounds that possess antibacterial and antibiofilm properties. This study evaluated the effect of chitosan-arginine nanoparticles (CS-Arg NPs) and sodium fluoride (NaF) on inhibiting . ' growth. After synthesizing CS-Arg NPs, their size, morphology, and chemical structure were evaluated. The broth microdilution method determined the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CS-Arg NPs and NaF. The combined antibacterial and antibiofilm effect of CS-Arg NPs and NaF was assessed using the checkerboard method. The CS-Arg NPs had an average size of 269.9 nm with a zeta potential of +38.3 mV. The MIC of . for CS-Arg NPs and NaF was 312 µg/mL and 625 µg/mL, respectively, and the MBC for these NPs and NaF was 625 µg/mL and 2500 µg/mL, respectively. The fractional inhibitory concentration index (FICI) of the combination of CS-Arg NPs and NaF showed an additive effect (FICI = 1). The inhibitory effect of different concentrations of CS-Arg NPs and NaF, alone or in combination, on biofilm formation in the studied strain ranged from approximately 12% to 81%. This study demonstrated that CS-Arg NPs have antibacterial and antibiofilm properties against . , and their combination with NaF can enhance these antibacterial effects. These findings suggest that CS-Arg NPs and NaF, as a novel combination, could effectively develop oral hygiene products.
龋齿是最常见的慢性病之一。它是由于代谢活动在牙齿表面形成细菌生物膜而引发的。（. ）是与龋齿发展相关的关键病原体。随着细菌对传统治疗的耐药性增加，人们越来越关注使用具有抗菌和抗生物膜特性的新型化合物。本研究评估了壳聚糖 - 精氨酸纳米颗粒（CS - Arg NPs）和氟化钠（NaF）对抑制（. ）生长的作用。合成CS - Arg NPs后，对其尺寸、形态和化学结构进行了评估。采用肉汤微量稀释法测定CS - Arg NPs和NaF的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。使用棋盘法评估CS - Arg NPs和NaF联合的抗菌和抗生物膜效果。CS - Arg NPs的平均尺寸为269.9 nm，zeta电位为 +38.3 mV。（. ）对CS - Arg NPs和NaF的MIC分别为312 µg/mL和625 µg/mL，这些纳米颗粒和NaF的MBC分别为625 µg/mL和2500 µg/mL。CS - Arg NPs和NaF组合的分数抑菌浓度指数（FICI）显示出相加作用（FICI = 1）。不同浓度的CS - Arg NPs和NaF单独或联合使用对所研究菌株生物膜形成的抑制作用范围约为12%至81%。本研究表明，CS - Arg NPs对（. ）具有抗菌和抗生物膜特性，它们与NaF联合可增强这些抗菌效果。这些发现表明，CS - Arg NPs和NaF作为一种新型组合，可有效开发口腔卫生产品。
