Puriastuti Alifia Candra, Maramis Margarita Maria, Annas Jimmy Yanuar, I'tishom Reny, Srirejeki Purwo
Midwifery Department, Faculty of Medicine, State University of Malang, Jalan Semarang No. 5, Malang, Indonesia.
Reproductive Health Science, Postgraduate Programme, Medical Faculty, Jalan Prof. Dr. Moestopo No. 47, Universitas Airlangga Surabaya, Indonesia.
Int J Reprod Biomed. 2025 Jun 10;23(4):303-312. doi: 10.18502/ijrm.v23i4.18782. eCollection 2025 Apr.
The chronic unpredictable mild stress (CUMS) model involves applying mild stressors over a prolonged period, that can induce oxidative stress. Oxidative stress disrupts normal cellular functions, leading to reduced survival of antral follicles through increased glucocorticoids.
This study aims to identify the effect of stress on the regulation of glucocorticoid hormones and its impact on impaired antral follicles. Specifically, it focuses on the number of antral follicles using the CUMS model in female rats.
16 female Wistar rats (5-6 months, 300-350 gr) were divided into 2 groups (n = 8/each), the control and the CUMS model. 24 hr after the last treatment, they were eter euthanized, a blood sample was taken from the intracardial to measure corticosteroid levels using the enzyme-linked immunoassay (ELISA) method, ovarian preparations were made, and then the histological sections were observed.
After 22 days of CUMS, a significant difference was observed in corticosterone levels (p = 0.03), but no significant difference was observed in the number of antral follicles between the 2 groups (p = 0.57). However, histological analysis indicated substantial differences. The control group's ovaries exhibited a higher proportion of healthy antral follicles.
CUMS increases glucocorticoids, which in turn causes a decrease in the number of antral follicles. This happens through 2 mechanisms: suppression of gonadotrophin-releasing hormone and direct effects on the ovaries that elevate granulosa cell apoptosis and follicular atresia, ultimately leading to a reduction in antral follicles.
慢性不可预测轻度应激（CUMS）模型涉及在较长时间内施加轻度应激源，这会诱导氧化应激。氧化应激会破坏正常细胞功能，通过增加糖皮质激素导致窦卵泡存活率降低。
本研究旨在确定应激对糖皮质激素调节的影响及其对受损窦卵泡的影响。具体而言，它聚焦于使用CUMS模型的雌性大鼠的窦卵泡数量。
16只雌性Wistar大鼠（5 - 6个月，300 - 350克）分为2组（每组n = 8），即对照组和CUMS模型组。最后一次处理24小时后，将它们安乐死，从心脏采集血样，使用酶联免疫吸附测定（ELISA）法测量皮质类固醇水平，制作卵巢标本，然后观察组织学切片。
CUMS处理22天后，观察到皮质酮水平有显著差异（p = 0.03），但两组之间窦卵泡数量无显著差异（p = 0.57）。然而，组织学分析表明存在实质性差异。对照组卵巢中健康窦卵泡的比例更高。
CUMS会增加糖皮质激素，进而导致窦卵泡数量减少。这通过两种机制发生：抑制促性腺激素释放激素以及对卵巢产生直接影响，从而提高颗粒细胞凋亡和卵泡闭锁，最终导致窦卵泡减少。
