Chakraborty Debanjali, Ghosh Surajit, Mandal Ahamadul Hoque, Roy Triparna, Sadhu Auroshree, Paria Dipanwita Sarkar, Saha Nimai Chandra, Sopjani Mentor, Saha Shubhajit, Faggio Caterina
Ecotoxicology Research Laboratory, Department of Zoology, The University of Burdwan, Burdwan, India.
Department of Zoology, Bethune College, Kolkata, West Bengal 700006, India.
Environ Toxicol Pharmacol. 2025 Sep;118:104778. doi: 10.1016/j.etap.2025.104778. Epub 2025 Aug 5.
In this study, the acute and sublethal toxicity of SDBS was assessed using Tubifex tubifex. In an acute toxicity bioassay, the 96-hour LC₅₀ value for SDBS was determined to be 13.358 mg/L. The survival probability of the test organism under elevated SDBS concentrations was further assessed using General Unified Threshold models of Survival (GUTS), where the GUTS-Individual Tolerance (IT) model was applied over the GUTS-Stochastic Death (SD) model. T. tubifex was exposed for 14 days to concentrations equivalent to 10 % and 20 % of the 96-hour LC₅₀ value (1.33 mg/L and 2.67 mg/L). Significant alterations were observed in total protein content, antioxidant enzyme activities, and histopathological markers. Specifically, levels of proteins, superoxide dismutase (SOD), and catalase (CAT) decreased, while glutathione S-transferase (GST), glutathione peroxidase (GPx), and malondialdehyde (MDA) increased, indicating oxidative stress. These findings suggest exposure to sublethal levels of SDBS may compromise physiological integrity and antioxidative defense mechanisms in invertebrates.
在本研究中，使用颤蚓对十二烷基苯磺酸钠（SDBS）的急性和亚致死毒性进行了评估。在急性毒性生物测定中，确定SDBS的96小时半数致死浓度（LC₅₀）值为13.358毫克/升。使用通用生存阈值模型（GUTS）进一步评估了受试生物在升高的SDBS浓度下的生存概率，其中GUTS个体耐受性（IT）模型优于GUTS随机死亡（SD）模型。将颤蚓暴露于相当于96小时LC₅₀值10%和20%的浓度（1.33毫克/升和2.67毫克/升）下14天。观察到总蛋白含量、抗氧化酶活性和组织病理学标志物有显著变化。具体而言，蛋白质、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）水平下降，而谷胱甘肽S-转移酶（GST）、谷胱甘肽过氧化物酶（GPx）和丙二醛（MDA）增加，表明存在氧化应激。这些发现表明，暴露于亚致死水平的SDBS可能会损害无脊椎动物的生理完整性和抗氧化防御机制。
