Park Jae B, Peters Renee
USDA, ARS, BHNRC, Diet, Genomics, and Immunology Laboratory, Bldg. 307C, Rm. 227, Beltsville, MD, 20705, USA.
Sci Rep. 2025 Aug 6;15(1):28806. doi: 10.1038/s41598-025-13590-3.
Coffee containing javamide I/II (CCJ12) is commonly found in the market. However, no information is available about chemical composition of CCJ12 and in vivo effects on obesity. Therefore, in this paper, the composition of CCJ12 was analyzed by HPLC and LC/MS, and effects on bodyweight, metabolic (HDL, LDL, TG, leptin, adiponectin), cardiovascular risk (sE-selectin, C-reactive protein,), and inflammatory (MCP-1, TNF-alpha) factors were investigated in a rodent model. In CCJ12, > 700 compounds were identified by LC/MS and the amounts of javamide I/II, caffeine and chlorogenic acids were quantified by HPLC. For the animal study, rats were placed into three groups (each n = 10); (CG group (a control diet with water), FG group (a high fat diet with water), and FCG group (a high fat diet with CCJ12)) and the study was conducted for 20 weeks. The data showed no significant differences in water/food intakes between all three groups. However, the FG and FCG groups showed weight gain, in comparison to the CG group (P < 0.05). Also, the FG and FCG groups showed higher levels of LDL, TG, and leptin than the CG group (P < 0.05). However, no significant differences were found in bodyweight, HDL, LDL, TG, leptin, and adiponectin levels between the FG and FCG groups. Also, no significant differences were noted in sE-selectin, C-reactive protein, and MCP-1 levels between the FG and FCG groups. However, TNF-alpha level was found to be down in the FCG group, in comparison to the FG group (P < 0.05). Our study suggests that CCJ12 may have no adverse effects on bodyweight, HDL, LDL, TG, adiponectin, leptin, sE-selectin, C-reactive protein, and MCP-1, but may have a beneficial effect on TNF-alpha in rats fed a high fat diet.
市场上常见含爪哇酰胺I/II的咖啡（CCJ12）。然而，关于CCJ12的化学成分及其对肥胖的体内影响尚无相关信息。因此，本文通过高效液相色谱法（HPLC）和液相色谱-质谱联用（LC/MS）分析了CCJ12的成分，并在啮齿动物模型中研究了其对体重、代谢（高密度脂蛋白、低密度脂蛋白、甘油三酯、瘦素、脂联素）、心血管风险（可溶性E选择素、C反应蛋白）和炎症（单核细胞趋化蛋白-1、肿瘤坏死因子-α）因子的影响。在CCJ12中，通过LC/MS鉴定出700多种化合物，并通过HPLC对爪哇酰胺I/II、咖啡因和绿原酸的含量进行了定量。在动物研究中，将大鼠分为三组（每组n = 10）；（CG组（以水为对照饮食）、FG组（以水为高脂饮食）和FCG组（以CCJ12为高脂饮食）），研究持续20周。数据显示，三组之间的水/食物摄入量无显著差异。然而，与CG组相比，FG组和FCG组体重增加（P < 0.05）。此外，FG组和FCG组的低密度脂蛋白、甘油三酯和瘦素水平高于CG组（P < 0.05）。然而，FG组和FCG组之间的体重、高密度脂蛋白、低密度脂蛋白、甘油三酯、瘦素和脂联素水平无显著差异。此外，FG组和FCG组之间的可溶性E选择素、C反应蛋白和单核细胞趋化蛋白-1水平也无显著差异。然而，与FG组相比，FCG组的肿瘤坏死因子-α水平降低（P < 0.05）。我们的研究表明，CCJ12可能对体重、高密度脂蛋白、低密度脂蛋白、甘油三酯、脂联素、瘦素、可溶性E选择素、C反应蛋白和单核细胞趋化蛋白-1没有不良影响，但可能对高脂饮食喂养的大鼠的肿瘤坏死因子-α有有益影响。
