Zhou Xiaochun, Zhao Jing, Wang Jianqin, He Kaiying, Du Hongxuan, You Qicai, Gu Wenjiao, Niu Haiyu, Jin Qiaoying, Kong Yuke, Tang Futian
The Second Hospital and Clinical Medical School, Lanzhou University, Lanzhou, Gansu, 730000, People's Republic of China.
Department of Cardiovascular Disease, Lanzhou University Second Hospital, Lanzhou, Gansu, 730000, People's Republic of China.
BMC Nephrol. 2025 Aug 6;26(1):440. doi: 10.1186/s12882-025-04360-4.
Diabetic kidney disease (DKD) is a frequent microvascular complication of diabetes and the predominant cause of end-stage renal disease worldwide. Dysregulated microRNA (miRNA) expression contributes to DKD pathogenesis. This study aimed to determine the clinical significance of serum exosomal miR-1207-5p expression in type-2 DKD.
Serum exosomes were isolated from 51 DKD patients stratified into low-, medium-, high-, and extremely high-risk groups and 11 control individuals. Exsosomal miR-1207-5p expression was determined by real-time-quantitative polymerase chain reaction (RT-qPCR), and its relationship with the patient's clinical records was explored. Bioinformatics analyses were performed to determine miR-1207-5p target genes using tools available online. Datasets obtained from the Gene Expression Omnibus (GEO) database were used to validate the experimental results.
miR-1207-5p was downregulated in the DKD patients compared to the controls, and this downregulation was the most prominent in the high-risk group. Correlation analysis revealed inverse associations between miR-1207-5p and parameters of renal dysfunction. Multivariate logistic regression indicated that miR-1207-5p may confer protection against DKD progression. Receiver operating characteristic (ROC) curve analysis demonstrated the ability of exosomal miR-1207-5p to distinguish low- versus high-/extremely high-risk DKD. Bioinformatics approaches identified a miR-1207-5p-mediated competing endogenous RNA (ceRNA) network with connections to pathogenic pathways.
Serum exosomal miR-1207-5p holds promise as a noninvasive biomarker for assessing DKD progression risk and improving the diagnosis and prognosis of affected patients.
糖尿病肾病（DKD）是糖尿病常见的微血管并发症，也是全球终末期肾病的主要原因。微小RNA（miRNA）表达失调在DKD发病机制中起作用。本研究旨在确定血清外泌体miR-1207-5p表达在2型DKD中的临床意义。
从51例DKD患者（分为低、中、高和极高风险组）和11例对照个体中分离血清外泌体。通过实时定量聚合酶链反应（RT-qPCR）测定外泌体miR-1207-5p表达，并探讨其与患者临床记录的关系。利用在线工具进行生物信息学分析以确定miR-1207-5p靶基因。从基因表达综合数据库（GEO）获得的数据集用于验证实验结果。
与对照组相比，DKD患者中miR-1207-5p表达下调，且这种下调在高风险组中最为明显。相关性分析显示miR-1207-5p与肾功能不全参数呈负相关。多因素逻辑回归表明miR-1207-5p可能对DKD进展具有保护作用。受试者工作特征（ROC）曲线分析表明外泌体miR-1207-5p能够区分低风险与高/极高风险DKD。生物信息学方法鉴定出一个与致病途径相关联的miR-1207-5p介导的竞争性内源RNA（ceRNA）网络。
血清外泌体miR-1207-5p有望作为一种无创生物标志物，用于评估DKD进展风险并改善受影响患者的诊断和预后。
