Han Chao, Zhang Yiping, Liang Hengyu, Chen Minliang, Li Jiahuang, Hua Zichun
The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.
Henan Joincare Biopharma Research Institute Co. Ltd, Jinyuan Street 8, Jiaozuo, 454000, People's Republic of China.
Sci Data. 2025 Aug 7;12(1):1378. doi: 10.1038/s41597-025-05645-x.
Acremonium chrysogenum is a notable filamentous fungus recognized for its essential contribution to the pharmaceutical sector through the biosynthesis of cephalosporin C (CPC). CPC functions as a key intermediate in the biosynthesis of β-lactam antibiotics, which are employed to combat bacterial infections. This study successfully generated a telomere-to-telomere (T2T) chromosome-scale genome sequence for A. chrysogenum, combining BGI short reads, PacBio HiFi long reads, and Hi-C technology. This genome sequence contained eight complete chromosomes (29.00 Mb) and a circular mitochondrial genome (27.27 kb), featuring an N50 length of 3.87 Mb. Repetitive elements accounted for 9.65% of genomic content, and a total of 7,745 genes involved in protein coding were annotated. This well-assembled reference genome of A. chrysogenum serves as an important foundation for elucidating the biosynthetic pathway of cephalosporin C and for molecular breeding. Furthermore, it offers valuable insights into chromosome organization, genome evolution, and regulatory mechanisms, facilitating future advancements in antibiotic research and fungal biotechnology.
产黄顶头孢霉是一种著名的丝状真菌，因其通过头孢菌素C（CPC）的生物合成对制药行业做出的重要贡献而闻名。CPC是β-内酰胺抗生素生物合成中的关键中间体，β-内酰胺抗生素用于对抗细菌感染。本研究结合了华大基因的短读长、PacBio HiFi长读长和Hi-C技术，成功生成了产黄顶头孢霉的端粒到端粒（T2T）染色体规模的基因组序列。该基因组序列包含8条完整染色体（29.00 Mb）和一个环状线粒体基因组（27.27 kb），N50长度为3.87 Mb。重复元件占基因组含量的9.65%，共注释了7745个参与蛋白质编码的基因。产黄顶头孢霉这个组装良好的参考基因组是阐明头孢菌素C生物合成途径和分子育种的重要基础。此外，它为染色体组织、基因组进化和调控机制提供了有价值的见解，有助于抗生素研究和真菌生物技术的未来进展。
