Han Gil, Cheon Wonsu, Heo Yunjeong, Lee Chang Soo
Fungi Research Division, Biological Resources Research Department, Nakdonggang National Institute of Biological Resources, Sangju 37242, Korea.
Plant Pathol J. 2025 Aug;41(4):484-497. doi: 10.5423/PPJ.OA.03.2025.0041. Epub 2025 Aug 1.
Colletotrichum and Fusarium are globally important plant-pathogenic fungi that cause serious diseases in chili pepper and other crops, leading to substantial yield losses due to their broad host range, environmental persistence, and the limited effectiveness of chemical control, thereby highlighting the need for sustainable alternatives such as biological control. We investigate the biological control and plant growth-promoting potential of Burkholderia vietnamiensis FBCC-B8049, isolated from freshwater environments. The strain exhibited significant antifungal activity against Colletotrichum gloeosporioides, Colletotrichum acutatum, and Fusarium oxysporum in dual-culture assays, with stronger effects against Colletotrichum species. The inhibition was likely due to direct antagonism and volatile organic compounds (VOCs) produced by FBCC-B8049, which were particularly effective against Colletotrichum species. Additionally, FBCC-B8049 demonstrated plant growth-promoting activities including siderophore production for iron acqusition, phosphate solubilization for enhanced nutrient availability, and indole-3-acetic acid synthesis to promote root development. These combined activities enhance nutrient availability and promote seed germination and seedling growth in chili pepper. Ex vivo assays further revealed the effectiveness of FBCC-B8049 in suppressing anthracnose disease on pepper fruits through both direct application and bacterial VOCs emission. Phylogenetic analysis of 16S rRNA and recA gene sequences positioned FBCC-B8049 closely to Burkholderia vietnamiensis, a known plant growth-promoting rhizobacterium. These findings highlight FBCC-B8049 as a promising candidate for sustainable agricultural applications.
炭疽菌属和镰刀菌属是全球重要的植物病原真菌，可在辣椒和其他作物上引发严重病害，因其寄主范围广、环境持久性强以及化学防治效果有限而导致大量产量损失，从而凸显了对生物防治等可持续替代方法的需求。我们研究了从淡水环境中分离出的越南伯克霍尔德氏菌FBCC-B8049的生物防治和促进植物生长的潜力。在双培养试验中，该菌株对胶孢炭疽菌、尖孢炭疽菌和尖孢镰刀菌表现出显著的抗真菌活性，对炭疽菌属的抑制作用更强。这种抑制可能是由于FBCC-B8049产生的直接拮抗作用和挥发性有机化合物（VOCs），这些物质对炭疽菌属特别有效。此外，FBCC-B8049还表现出促进植物生长的活性，包括产生铁载体以获取铁、溶解磷酸盐以提高养分有效性以及合成吲哚-3-乙酸以促进根系发育。这些综合活性提高了养分有效性，促进了辣椒种子萌发和幼苗生长。离体试验进一步揭示了FBCC-B8049通过直接应用和细菌VOCs排放抑制辣椒果实炭疽病的有效性。对16S rRNA和recA基因序列的系统发育分析表明，FBCC-B8049与已知的促进植物生长的根际细菌越南伯克霍尔德氏菌密切相关。这些发现突出了FBCC-B8049作为可持续农业应用的有前景候选菌株。
