Cheng Xinyu, Zhang Xuelin, Ni Jialei, Ding Houkang, Ma Weiming, Wang Dong
College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong, China.
Anim Sci J. 2025 Jan-Dec;96(1):e70096. doi: 10.1111/asj.70096.
This study evaluated the protective effects of dietary α-linolenic acid (ALA) on liver inflammation and oxidative stress induced by lipopolysaccharide (LPS) in broilers. A total of 256 AA broilers were randomly allocated to 4 groups (Con, LPS, LPS + ALA400, and LPS + ALA600) with 8 replicates. On d 20, the Con group broilers were intraperitoneally injected with sterile saline, and the other groups were injected with LPS (0.5 mg/kg of body weight). The trial lasted for 21 days. Results indicated that there were no significant differences in growth performance across the groups. ALA supplementation at 600 mg/kg significantly improved the levels of low-density lipoprotein cholesterol, serum and liver catalase, total antioxidant capacity, and superoxide dismutase while decreasing the levels of aspartate aminotransferase, alkaline phosphatase and malondialdehyde as compared with the LPS group broilers. Moreover, dietary supplementation with 600 mg/kg ALA markedly attenuated LPS-induced inflammation and oxidative stress by downregulating the expression of pro-inflammatory cytokines (IL-1β, TNF-α, and IFN-γ) and the TLR4/NF-κB pathway, while upregulating the expression of antioxidant enzymes (CAT, GSH-Px, SOD1) and the Nrf2/HO-1 pathway. In conclusion, ALA supplementation mitigates LPS-induced liver inflammation and oxidative stress in broilers by enhancing liver antioxidant capacity and modulating TLR4/NF-κB and Nrf2/HO-1 signaling pathways.
本研究评估了日粮α-亚麻酸（ALA）对肉仔鸡脂多糖（LPS）诱导的肝脏炎症和氧化应激的保护作用。将256只AA肉仔鸡随机分为4组（对照组、LPS组、LPS + ALA400组和LPS + ALA600组），每组8个重复。在第20天，对照组肉仔鸡腹腔注射无菌生理盐水，其他组注射LPS（0.5 mg/kg体重）。试验持续21天。结果表明，各组生长性能无显著差异。与LPS组肉仔鸡相比，添加600 mg/kg ALA显著提高了低密度脂蛋白胆固醇、血清和肝脏过氧化氢酶、总抗氧化能力和超氧化物歧化酶水平，同时降低了天冬氨酸转氨酶、碱性磷酸酶和丙二醛水平。此外，日粮添加600 mg/kg ALA通过下调促炎细胞因子（IL-1β、TNF-α和IFN-γ）和TLR4/NF-κB信号通路的表达，同时上调抗氧化酶（CAT、GSH-Px、SOD1）和Nrf2/HO-1信号通路的表达，显著减轻了LPS诱导的炎症和氧化应激。总之，添加ALA通过增强肝脏抗氧化能力和调节TLR4/NF-κB及Nrf2/HO-1信号通路减轻了LPS诱导的肉仔鸡肝脏炎症和氧化应激。
