Núñez-Martínez Manuel, Dong Jinyi, García Isabel, De Busschere Bjorn, Claes Nathalie, Bals Sara, Liz-Marzán Luis M
CIC biomaGUNE, Basque Research and Technology Alliance (BRTA) 20014 Donostia-San Sebastián Spain
CAS Key Laboratory of Nano-Bio Interface, Suzhou Key Laboratory of Functional Molecular Imaging Technology, Division of Nanobiomedicine and i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences Suzhou 215123 China.
Chem Sci. 2025 Jul 31. doi: 10.1039/d5sc04459f.
Motivated by potential applications in fields such as medicine or materials science, various methodologies have been developed for the preparation of so-called spherical nucleic acids, based on oligonucleotides and metal nanoparticles. Despite optimization through various parameters such as loading efficiency or nanoconjugate stability, much remains to be known regarding the actual conformations of oligonucleotides and their interactions with the nanoparticle surface. We employed a combination of spectroscopic techniques and liquid transmission electron microscopy to analyze the interactions and conformations adopted by polyAdenine (polyA) and polyThymine (polyT) chains in contact with gold nanoparticles (AuNPs). These studies revealed the presence of AuNP@polyA dimers, with polyA strands forming duplexes, whereas polyT forms isolated strands on the AuNPs. The presence or absence of polyA duplexes on AuNPs can be modulated by external stimuli such as temperature or NaCl. This study contributes to understanding the interactions and secondary structure of oligonucleotides on AuNPs.
受医学或材料科学等领域潜在应用的推动，基于寡核苷酸和金属纳米颗粒，已开发出各种制备所谓球形核酸的方法。尽管通过诸如负载效率或纳米缀合物稳定性等各种参数进行了优化，但关于寡核苷酸的实际构象及其与纳米颗粒表面的相互作用仍有许多未知之处。我们采用光谱技术和液体透射电子显微镜相结合的方法，分析了与金纳米颗粒（AuNP）接触的聚腺嘌呤（polyA）和聚胸腺嘧啶（polyT）链所采用的相互作用和构象。这些研究揭示了AuNP@polyA二聚体的存在，其中polyA链形成双链体，而polyT在AuNP上形成孤立的链。AuNP上polyA双链体的存在与否可通过温度或NaCl等外部刺激进行调节。这项研究有助于理解寡核苷酸在AuNP上的相互作用和二级结构。
