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一种新型糖基工程重组候选疫苗对绵羊捻转血矛线虫的安全性和有效性

Safety and efficacy of a novel glycoengineered recombinant vaccine candidate against Haemonchus contortus in sheep.

作者信息

Sajovitz-Grohmann Floriana, Adduci Isabella, Werling Dirk, Wiedermann Sandra, Wortha Licha N, Prole Bojan, Zlöbl Julia, Elster Jolina, Tichy Alexander, Joachim Anja, Wittek Thomas, Hinney Barbara, Yan Shi, Lichtmannsperger Katharina

机构信息

Clinical Centre for Ruminant and Camelid Medicine, Clinical Department for Farm Animals and Food System Science, University of Veterinary Medicine Vienna, Vienna, Austria.

Institute of Parasitology, Department of Biological Sciences and Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria.

出版信息

NPJ Vaccines. 2025 Aug 11;10(1):190. doi: 10.1038/s41541-025-01249-z.

DOI:10.1038/s41541-025-01249-z
PMID:40790301
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12339958/
Abstract

Haemonchus contortus is a highly pathogenic nematode in small ruminants, causing major production losses and animal welfare concerns. With increasing anthelmintic resistance, vaccination offers a more sustainable control strategy. This study evaluated a novel glycoengineered vaccine produced in Hi5 insect cells in a randomized, controlled trial including Jura × Lacaune sheep. Animals received either Barbervax (BVAX), the glycoengineered vaccine (GEA) composed of five antigens (H11, H11-1, H11-2, H11-4 and GA1), its non-glycoengineered counterpart (NEA), or served as controls. Over 16 weeks, clinical, immunological, and parasitological data were collected. GEA reduced fecal egg counts by 81.09% and worm burden by 25.36%, showing a lower degree of anemia compared to NEA and control. All vaccinated groups exhibited elevated IgG/IgE responses. The results underscore the importance of glycoengineering in achieving protective immunity against H. contortus, supporting the development of highly effective recombinant vaccines against this and other parasitic worms in the future.

摘要

捻转血矛线虫是小型反刍动物中一种高致病性线虫，会导致重大的生产损失并引发动物福利问题。随着抗蠕虫药耐药性的增加，疫苗接种提供了一种更可持续的控制策略。本研究在一项包括汝拉×拉库内绵羊的随机对照试验中，评估了在Hi5昆虫细胞中生产的一种新型糖基工程疫苗。动物分别接种Barbervax（BVAX）、由五种抗原（H11、H11-1、H11-2、H11-4和GA1）组成的糖基工程疫苗（GEA）、其非糖基工程对应物（NEA），或作为对照。在16周内，收集了临床、免疫学和寄生虫学数据。GEA使粪便虫卵计数降低了81.09%，虫负荷降低了25.36%，与NEA和对照组相比，贫血程度更低。所有接种疫苗的组均表现出IgG/IgE反应升高。结果强调了糖基工程在实现针对捻转血矛线虫的保护性免疫方面的重要性，为未来开发针对这种及其他寄生蠕虫的高效重组疫苗提供了支持。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7184/12339958/b730ad61a8bd/41541_2025_1249_Fig11_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7184/12339958/f6da9941154e/41541_2025_1249_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7184/12339958/d9ecb9943ca1/41541_2025_1249_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7184/12339958/c3668acfe07d/41541_2025_1249_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7184/12339958/8f49dc861354/41541_2025_1249_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7184/12339958/5c2eadc02a00/41541_2025_1249_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7184/12339958/9d7054172642/41541_2025_1249_Fig10_HTML.jpg
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