Zhang Qian, Zhou Xiaogang, Hao Xianglin, Sun Qi, Zhang Gang, Xu Shuai
Department of Stomatology, The Second Affiliated Hospital, Third Military Medical University (Army Medical University), Chongqing, China.
Department of Respiratory and Critical Care Medicine, General Hospital of Central Theater Command of the Chinese People's Liberation Army, Wuhan, China.
Transl Cancer Res. 2025 Jul 30;14(7):3905-3919. doi: 10.21037/tcr-24-2130. Epub 2025 Jul 27.
Tandem C2 domains, nuclear (TC2N) is a recently identified tumor-associated gene that has been confirmed to play important roles in various malignancies, including lung cancer, breast cancer, gastric cancer, glioma, and liver cancer. However, its biological functions and mechanisms in head and neck squamous cell carcinoma (HNSCC) remain unclear. In this study, we aimed to analyze the expression and methylation status of TC2N using public databases and preliminarily investigate the effects of TC2N on the malignant phenotypes of HNSCC cells and its potential mechanisms.
Data from The Cancer Genome Atlas (TCGA) and multiple Gene Expression Omnibus (GEO) databases were analyzed to assess the expression levels of TC2N in HNSCC. Functional and signaling pathway analyses were conducted both and to evaluate the impact of TC2N on cell growth, invasion, and apoptosis. The role of TC2N in promoting cancer through mutant p53-mediated signaling was also explored.
The study found that TC2N was downregulated in HNSCC and its expression was correlated with the degree of promoter methylation. Both and experiments demonstrated that TC2N can stimulate cell growth and invasion, and inhibit apoptosis via mutant p53-R175-mediated pro-cancer signals.
This study reports the expression pattern of TC2N in HNSCC and provides preliminary insights into the biological functions and mechanisms of this gene in this type of cancer, laying the experimental foundation for investigating the role and mechanism of TC2N in HNSCC.
串联C2结构域，核内（TC2N）是一种最近鉴定出的肿瘤相关基因，已证实其在包括肺癌、乳腺癌、胃癌、神经胶质瘤和肝癌在内的各种恶性肿瘤中发挥重要作用。然而，其在头颈部鳞状细胞癌（HNSCC）中的生物学功能和机制仍不清楚。在本研究中，我们旨在利用公共数据库分析TC2N的表达和甲基化状态，并初步研究TC2N对HNSCC细胞恶性表型的影响及其潜在机制。
分析来自癌症基因组图谱（TCGA）和多个基因表达综合数据库（GEO）的数据，以评估HNSCC中TC2N的表达水平。进行功能和信号通路分析，以评估TC2N对细胞生长、侵袭和凋亡的影响。还探讨了TC2N通过突变型p53介导的信号通路促进癌症的作用。
研究发现，TC2N在HNSCC中表达下调，其表达与启动子甲基化程度相关。实验均表明，TC2N可通过突变型p53-R175介导的促癌信号刺激细胞生长和侵袭，并抑制凋亡。
本研究报道了TC2N在HNSCC中的表达模式，并对该基因在这类癌症中的生物学功能和机制提供了初步见解，为研究TC2N在HNSCC中的作用和机制奠定了实验基础。
