Jeong Yeon-Su, Lim Seong-Keun, Kim You-Jin, Lee Seung-Yeol, Jung Hee-Young
Department of Plant Medicine, Kyungpook National University, Daegu, Republic of Korea.
Institute of Plant Medicine, Kyungpook National University, Daegu, Republic of Korea.
Mycobiology. 2025 Aug 7;53(5):629-636. doi: 10.1080/12298093.2025.2535782. eCollection 2025.
Strain KNUF-23-295 was obtained from a soil sample collected from Nami-myeon, Geumsan-gun, Chungcheongnam-do Province, South Korea, during a survey of fungal diversity in Korean soils. Molecular analysis based on internal transcribed spacer (ITS) regions revealed 97.3% sequence similarity to but comparatively lower similarities to other species in the genus , including (94.6%) and (94.4%). These results suggest that the strain is phylogenetically affiliated with the genus . Furthermore, strain KNUF-23-295 exhibited distinct cultural and morphological characteristics that differentiated it from closely related species. It was characterized by the production of a pink pigment on potato dextrose agar, asymmetrical colonies on cornmeal agar, shorter and narrower conidiophores (6.5-20.0 × 2.5-3.9 μm), bottle-shaped phialides (8.4-20.9 × 2.0-4.7 μm), and a distinctive conidial arrangement consisting of solitary conidia, short chains (two to five), long chains (up to 15), and aggregates. Two phylogenetic trees based on a concatenated dataset of ITS, , , and sequences consistently placed strain KNUF-23-295 as a distinct and well-supported lineage in the genus , providing strong phylogenetic evidence for its novelty. The morphological characteristics and multilocus phylogenetic data indicate that strain KNUF-23-295 is a novel species, for which the name sp. nov. is proposed.
菌株KNUF-23-295是在对韩国土壤真菌多样性进行调查期间，从韩国忠清南道锦山郡南面上里采集的土壤样本中获得的。基于内部转录间隔区（ITS）区域的分子分析显示，其与[具体物种名称1]的序列相似性为97.3%，但与该属其他物种的相似性相对较低，包括[具体物种名称2]（94.6%）和[具体物种名称3]（94.4%）。这些结果表明，该菌株在系统发育上隶属于[属名]属。此外，菌株KNUF-23-295表现出独特的培养和形态特征，使其与近缘物种区分开来。其特征在于在马铃薯葡萄糖琼脂上产生粉红色色素，在玉米粉琼脂上形成不对称菌落，分生孢子梗较短且较窄（6.5 - 20.0×2.5 - 3.9μm），瓶形瓶梗（8.4 - 20.9×2.0 - 4.7μm），以及由单个分生孢子、短链（2至5个）、长链（最多15个）和聚集体组成的独特分生孢子排列。基于ITS、[其他基因名称1]、[其他基因名称2]和[其他基因名称3]序列的串联数据集构建的两棵系统发育树一致地将菌株KNUF-23-295置于[属名]属中一个独特且得到充分支持的谱系中，为其新颖性提供了有力的系统发育证据。形态特征和多位点系统发育数据表明，菌株KNUF-23-295是一个新物种，为此提出新名称[新物种名称] sp. nov. 。
