Jones A W
J Anal Toxicol. 1985 Nov-Dec;9(6):246-50. doi: 10.1093/jat/9.6.246.
This paper describes a gas chromatographic method suitable for the analysis of low-molecular endogenous volatiles in human breath. The use of an on-column gas sampling device allowed serial determinations at 10-min intervals. With a flame ionization detector and Porapak Q as stationary phase, the four major endogenous breath volatiles were methanol, ethanol, acetone, and isoprene (2-methyl-1,3-butadiene). These same compounds were present in the breath of healthy individuals and abstinent alcoholics. In 10 healthy men, the breath concentration of endogenous ethanol, methanol, and acetone ranged from 0.07 to 0.39 microgram/L, 0.21 to 0.07 microgram/L, and 0.57 to 4.01 micrograms/L, respectively. When a man drank 5 g of exogenous ethanol, the blood concentration increased 200 times above the endogenous level. This small dose of ethanol was eliminated with a half-life of 16 min.
本文描述了一种适用于分析人体呼出气体中低分子内源性挥发物的气相色谱法。使用柱上进样气体采样装置可每隔10分钟进行连续测定。采用火焰离子化检测器并以Porapak Q作为固定相，呼出气体中的四种主要内源性挥发物为甲醇、乙醇、丙酮和异戊二烯（2-甲基-1,3-丁二烯）。这些相同的化合物存在于健康个体和戒酒者的呼出气体中。在10名健康男性中，内源性乙醇、甲醇和丙酮的呼出气体浓度分别为0.07至0.39微克/升、0.21至0.07微克/升和0.57至4.01微克/升。当一名男性摄入5克外源性乙醇时，血液浓度比内源性水平升高了200倍。这一小剂量的乙醇以16分钟的半衰期被消除。
