Jones A W, Skagerberg S, Yonekura T, Sato A
Department of Alcohol, University Hospital, Linköping, Sweden.
Pharmacol Toxicol. 1990 Jan;66(1):62-5. doi: 10.1111/j.1600-0773.1990.tb00704.x.
Ethanol was administered to 5 healthy volunteers by intravenous infusion to maintain a constant blood-alcohol concentration (BAC) of about 0.8 mg/ml (17.4 mmol/l) for 3-4 hr. Before starting the infusion and at 15 min. intervals thereafter, we analysed end-expired air by a highly sensitive gas chromatographic method. The presumed concentrations of ethanol and methanol in blood were estimated indirectly from measurements in the breath. The liquid/air partition coefficients of methanol were determined for whole blood, plasma, water, and corn-oil when dilute solutions were equilibrated at 34 degrees and 37 degrees. The results at 37 degrees were 2709 +/- 165, 3400 +/- 86, 2948 +/- 221 and 44.5 +/- 6.3 for whole-blood/air, water/air, plasma/air and corn-oil/air respectively. During infusion of ethanol, the concentrations of endogenous methanol in blood rose from being between 0.4-0.8 mg/l to reach between 1.2-3.4 mg/l with a substantial inter-subject variation in rate of increase. Our results confirm the existence of endogenous methanol in human blood and breath and demonstrate that the concentrations present can rise to abnormally high levels when the body is flooded with exogenous ethanol.
对5名健康志愿者进行静脉输注乙醇，以维持约0.8mg/ml（17.4mmol/l）的恒定血液酒精浓度（BAC）3 - 4小时。在开始输注前以及此后每隔15分钟，我们采用高灵敏度气相色谱法分析呼出的终末气体。血液中乙醇和甲醇的假定浓度通过呼气测量间接估算。当稀溶液在34℃和37℃达到平衡时，测定了全血、血浆、水和玉米油中甲醇的液/气分配系数。37℃时全血/气、水/气、血浆/气和玉米油/气的结果分别为2709±165、3400±86、2948±221和44.5±6.3。在输注乙醇期间，血液中内源性甲醇的浓度从0.4 - 0.8mg/l升至1.2 - 3.4mg/l，个体间的上升速率存在显著差异。我们的结果证实了人体血液和呼出气体中存在内源性甲醇，并表明当身体摄入大量外源性乙醇时，内源性甲醇的浓度可能会升至异常高水平。
