Hansell Emily J, Reynolds Kirsty M, Škarabot Jakob, James Lewis J, Clifford Tom, Thorley Josh
School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, UK.
NIHR Leicester Biomedical Research Centre, University Hospitals of Leicester NHS Trust and University of Leicester, Leicester, UK.
Eur J Appl Physiol. 2025 Aug 12. doi: 10.1007/s00421-025-05936-6.
To examine the effects of muscle-damaging exercise on urinary N-terminal fragments of titin (UTF) in males and females, and its association with markers of exercise-induced muscle damage.
27 males (n = 16) and females (n = 11) (height: 1.74 ± 0.10 m; body mass: 72.2 ± 11.4 kg; age: 22 ± 3 years) performed 200 eccentric contractions of the knee extensor on an isokinetic dynamometer. Urine and serum samples were collected pre-, post- and 48 h post-exercise to quantify UTF and creatine kinase (CK). Additionally, knee extensor maximal voluntary isometric force (MVIF), voluntary activation (VA), time to peak twitch (TTP), evoked maximal rate of force development (RFD), potentiated twitch force (Tw), and delayed-onset muscle soreness (DOMS) were recorded.
UTF (2.3 ± 1.8 to 3.3 ± 3.4 nmol/mg/dL) and CK (9.7 ± 4.8 to 14.5 ± 8.7 units/L) concentrations were elevated 48 h after exercise (p < 0.01). DOMS was greater at all post-exercise time points vs. pre-exercise (p < 0.01). MVIF, evoked RFD, VA, and Tw all decreased after exercise (p < 0.01). The pre- to 48 h post-exercise change in UTF strongly correlated with CK (r = 0.73; p < 0.01), TTP (r = -0.77; p < 0.01) and evoked RFD (r = -0.62; p < 0.01) and moderately correlated with MVIF (r = -0.45; p < 0.01). Moderate strength correlations were found between the pre- to 48 h post-change in CK with DOMS (r = 0.47; p = 0.03). There were no sex differences for any variables (p > 0.05).
UTF was similarly increased post- and 48 h post-exercise in males and females and was moderately to strongly correlated to CK and some markers of neuromuscular function, but not DOMS.
研究肌肉损伤性运动对男性和女性尿中肌联蛋白N端片段（UTF）的影响，及其与运动诱导的肌肉损伤标志物的关联。
27名男性（n = 16）和女性（n = 11）（身高：1.74±0.10米；体重：72.2±11.4千克；年龄：22±3岁）在等速测力计上进行200次膝关节伸肌离心收缩。在运动前、运动后和运动后48小时收集尿液和血清样本，以量化UTF和肌酸激酶（CK）。此外，记录膝关节伸肌最大自主等长肌力（MVIF）、自主激活（VA）、峰值抽搐时间（TTP）、诱发最大力量发展速率（RFD）、增强抽搐力（Tw）和延迟性肌肉酸痛（DOMS）。
运动后48小时，UTF（2.3±1.8至3.3±3.4纳摩尔/毫克/分升）和CK（9.7±4.8至14.5±8.7单位/升）浓度升高（p<0.01）。与运动前相比，所有运动后时间点的DOMS均更严重（p<0.01）。运动后MVIF、诱发RFD、VA和Tw均下降（p<0.01）。运动前至运动后48小时UTF的变化与CK（r = 0.73；p<0.01）、TTP（r = -0.77；p<0.01）和诱发RFD（r = -0.62；p<0.01）密切相关，与MVIF中度相关（r = -0.45；p<0.01）。CK运动前至运动后48小时的变化与DOMS之间存在中等强度相关性（r = 0.47；p = 0.03）。各变量均无性别差异（p>0.05）。
男性和女性运动后及运动后48小时UTF均有类似升高，且与CK及一些神经肌肉功能标志物呈中度至高度相关，但与DOMS无关。
