重编程白色脂肪细胞用于脂肪操纵移植（AMT）治疗。
Reprogramming White Fat Cells for Adipose Manipulation Transplantation (AMT) Therapy.
作者信息
An Kelly, Ito Yusuke, Ahituv Nadav
机构信息
Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA, USA.
Institute for Human Genetics, University of California San Francisco, San Francisco, CA, USA.
出版信息
Bio Protoc. 2025 Aug 5;15(15):e5405. doi: 10.21769/BioProtoc.5405.
Adipocytes are endocrine cells that function as the main energy storage in our body. They are commonly used in clinical procedures, including their removal via liposuction and transplantation in plastic surgery. Building on this, adipocytes can be used for ex vivo cellular manipulations, enabling therapeutic modifications that can provide beneficial clinical outcomes after transplantation. Here, we provide a detailed protocol on how to modify adipocytes and adipose organoids using CRISPR activation (CRISPRa), a technology termed adipose manipulation transplantation (AMT). Key features • This protocol is used to generate adipocytes and adipose organoids from human preadipocytes that can be genetically engineered for therapeutic purposes, including cancer metabolic therapy.
脂肪细胞是作为我们身体主要能量储存的内分泌细胞。它们常用于临床手术，包括通过抽脂术去除脂肪细胞以及在整形手术中进行移植。在此基础上，脂肪细胞可用于体外细胞操作，实现治疗性修饰，从而在移植后提供有益的临床结果。在这里，我们提供了一份详细的方案，介绍如何使用CRISPR激活（CRISPRa）技术（一种称为脂肪操作移植（AMT）的技术）对脂肪细胞和脂肪类器官进行修饰。关键特性 • 本方案用于从人类前脂肪细胞生成可进行基因工程改造以用于治疗目的（包括癌症代谢治疗）的脂肪细胞和脂肪类器官。
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