Brown R O, Gusman D, Basbaum A I, Mayeri E
Neuropeptides. 1985 Dec;6(6):517-26. doi: 10.1016/0143-4179(85)90113-1.
Electrophysiological and immunocytochemical techniques were used in the abdominal ganglion of Aplysia to identify neurons containing immunoreactive FMRFamide. Large numbers of neurons were immunoreactive for FMRFamide, including R2, L2, L3, L4, L5, L6, 2 cells tentatively identified as L12 and L13, and a previously unidentified cluster on the ventral surface of the right lower quadrant. There was also heavy labelling of fibers, often with beaded varicosities, throughout the neuropil, the cell layers, and the sheath overlying the ganglion. This data provides further evidence that FMRFamide is an important neurotransmitter in Aplysia. The demonstration of immunoreactive FMRFamide in the giant cholinergic neurons R2 and LP1(1) suggests that these well-studied and experimentally convenient cells use acetylcholine and an FMRFamide-like peptide as cotransmitters.
利用电生理和免疫细胞化学技术，在海兔的腹神经节中鉴定出含有免疫反应性FMRF酰胺的神经元。大量神经元对FMRF酰胺呈免疫反应性，包括R2、L2、L3、L4、L5、L6、2个暂定为L12和L13的细胞，以及右下象限腹侧表面一个先前未鉴定的细胞簇。在整个神经纤维网、细胞层以及覆盖神经节的鞘中，纤维也有大量标记，且常有串珠状膨大。这些数据进一步证明FMRF酰胺是海兔中的一种重要神经递质。在巨大胆碱能神经元R2和LP1(1)中发现免疫反应性FMRF酰胺，表明这些经过充分研究且实验操作方便的细胞使用乙酰胆碱和一种FMRF酰胺样肽作为共递质。
