曼氏血吸虫：单性感染未成熟雌虫的核酸合成，体外与完整雄虫及雄虫节段配对。

Schistosoma mansoni: nucleic acid synthesis in immature females from single-sex infections, paired in vitro with intact males and male segments.

Basch P F

Department of Family, Community and Preventive Medicine, Stanford University School of Medicine, CA 94305.

Comp Biochem Physiol B. 1988;90(2):389-92. doi: 10.1016/0305-0491(88)90093-4.

Abstract

The object of this study was to see whether stimulation of nucleic acid synthesis in immature females by male Schistosoma mansoni is mediated locally by contact, or is propagated systemically in the female. 2. Immature females perfused from single-sex animal infections were paired for one week in vitro with segments of males cut transversely into thirds; others were paired with intact males, or maintained without males; all were then incubated with [3H]-thymidine or tyrosine. 3. Washed females were bisected transversely and isotope uptake counted separately in the anterior and posterior halves. 4. The halves in contact with cut male segments showed significantly higher uptake of [3H]-thymidine than the non-contact halves, indicating increased DNA synthesis and cell division, but non-contact halves had greater uptake of [3H]-tyrosine. 5. Dot-blot hybridization with a female specific single stranded cDNA failed to detect production of the corresponding mRNA in females paired with male segments.

摘要

本研究的目的是观察曼氏血吸虫雄虫对未成熟雌虫核酸合成的刺激是通过局部接触介导，还是在雌虫体内全身性传播。2. 从未感染单性别的动物中取出未成熟雌虫，在体外与横向切成三段的雄虫节段配对一周；其他雌虫与完整雄虫配对，或不与雄虫接触单独培养；然后所有雌虫均与[3H] - 胸苷或酪氨酸一起孵育。3. 将洗净的雌虫横向切成两半，分别计数前半部分和后半部分的同位素摄取量。4. 与切割后的雄虫节段接触的半部分对[3H] - 胸苷的摄取量显著高于未接触的半部分，表明DNA合成和细胞分裂增加，但未接触的半部分对[3H] - 酪氨酸的摄取量更大。5. 用雌性特异性单链cDNA进行斑点印迹杂交，未能检测到与雄虫节段配对的雌虫中相应mRNA的产生。