比较序列分析作为研究mRNA二级结构的一种工具。
Comparative sequence analysis as a tool for studying the secondary structure of mRNAs.
作者信息
Browner M F, Lawrence C B
出版信息
Nucleic Acids Res. 1985 Dec 9;13(23):8645-60. doi: 10.1093/nar/13.23.8645.
Analysis of phylogenetically conserved secondary structure has been important in the development of models for the secondary structure of structural RNAs. In this paper, we apply this type of analysis to several families of informational RNAs to evaluate its usefulness in developing secondary structure models for mRNAs and mRNA precursors. We observed many conserved helices in all mRNA groups analyzed. Three criteria were used to identify potential helices which were not conserved solely because of coding sequence constraints, and may therefore be important for the structure and function of the RNA. These results suggest that this approach will be useful in deriving secondary structure models for informational RNAs when used in conjunction with other complementary techniques, and in designing experiments to determine the functional significance of conserved base pairing interactions.
对系统发育保守二级结构的分析在构建结构RNA二级结构模型的过程中发挥了重要作用。在本文中，我们将这类分析应用于几个信息RNA家族，以评估其在构建mRNA和mRNA前体二级结构模型方面的实用性。我们在所有分析的mRNA组中观察到许多保守螺旋。使用了三个标准来识别潜在的螺旋，这些螺旋并非仅仅由于编码序列限制而保守，因此可能对RNA的结构和功能很重要。这些结果表明，当与其他互补技术结合使用时，这种方法将有助于推导信息RNA的二级结构模型，并有助于设计实验来确定保守碱基配对相互作用的功能意义。
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