两种黑猩猩α-珠蛋白mRNA的结构与进化分析
Structural and evolutionary analysis of the two chimpanzee alpha-globin mRNAs.
作者信息
Liebhaber S A, Begley K A
出版信息
Nucleic Acids Res. 1983 Dec 20;11(24):8915-29. doi: 10.1093/nar/11.24.8915.
Two distinct alpha-globin mRNAs were detected in chimpanzee reticulocyte mRNA using a primer extension assay. DNA copies of these two mRNAs were cloned in the bacterial plasmid pBR322, and their sequence was determined. The two alpha-globin mRNAs have obvious structural homology to the two human alpha-globin mRNAs, alpha 1 and alpha 2. Comparison of the two chimpanzee alpha-globin mRNAs to each other and to their corresponding human counterparts revealed evidence of a recent gene conversion in the human alpha-globin complex and a marked heterogeneity in the rate of structural divergence within the alpha-globin gene.
使用引物延伸分析在黑猩猩网织红细胞mRNA中检测到两种不同的α-珠蛋白mRNA。这两种mRNA的DNA拷贝被克隆到细菌质粒pBR322中，并测定了它们的序列。这两种α-珠蛋白mRNA与两种人类α-珠蛋白mRNA（α1和α2）具有明显的结构同源性。将两种黑猩猩α-珠蛋白mRNA相互比较以及与相应的人类对应物比较，揭示了人类α-珠蛋白复合体中近期基因转换的证据以及α-珠蛋白基因内结构分歧速率的显著异质性。
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