Zhiponova Miroslava, Zehirov Grigor, Rusanov Krasimir, Rusanova Mila, Stefanova Miroslava, Ganeva Tsveta, Paunov Momchil, Ganeva Valentina, Mishev Kiril, Dobrev Petre I, Vaculíková Roberta, Motyka Václav, Yordanova Zhenya, Chaneva Ganka, Vassileva Valya
Faculty of Biology, Sofia University "St. Kliment Ohridski", 1164 Sofia, Bulgaria.
Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria.
Plants (Basel). 2025 Jul 24;14(15):2285. doi: 10.3390/plants14152285.
Light quality and duration profoundly influence the growth and productivity of plant species. This study investigated the effects of a blue-red LED light combination, known to induce flowering, on the physiological state and content of biologically active substances in catmint ( L.) grown under controlled in vitro conditions. White light (W) was used as a control and compared with two blue-red intensities: BR (high-intensity blue-red light) and BRS (low-intensity blue-red light or "BR with shadow"). BR-treated plants showed increased leaf area, mesophyll thickness, biomass and starch content but reduced levels of plastid pigments. BR also modified the oxidative state of plants by inducing lipid peroxidation while simultaneously activating ROS scavenging mechanisms and enhancing phenolic antioxidants. Interestingly, BR decreased the accumulation of the sp.-specific iridoid, nepetalactone. These effects appear to be regulated by the phytohormones auxin, abscisic acid and jasmonates. BRS treatment produced effects similar to the W control but led to increased plant height and reduced leaf area and thickness. Both BR and BRS regimes induced the accumulation of proteins and amino acids. We conclude that blue-red light can enhance the survival capacity of micropropagated during subsequent soil adaptation, suggesting that similar light pre-treatment could improve plant performance under stress conditions.
光质和光周期对植物物种的生长和生产力有深远影响。本研究调查了已知能诱导开花的红蓝LED光组合对在可控体外条件下生长的荆芥（荆芥属）生理状态和生物活性物质含量的影响。白光（W）用作对照，并与两种红蓝强度进行比较：BR（高强度红蓝光照）和BRS（低强度红蓝光照或“带阴影的BR”）。经BR处理的植株叶面积、叶肉厚度、生物量和淀粉含量增加，但质体色素水平降低。BR还通过诱导脂质过氧化作用改变植物的氧化状态，同时激活ROS清除机制并增强酚类抗氧化剂。有趣的是，BR降低了荆芥属特定环烯醚萜类化合物荆芥内酯的积累。这些效应似乎受植物激素生长素、脱落酸和茉莉酸酯调控。BRS处理产生的效果与W对照相似，但导致植株高度增加，叶面积和厚度减小。BR和BRS处理均诱导了蛋白质和氨基酸的积累。我们得出结论，红蓝光照可增强微繁殖荆芥在后续土壤适应过程中的存活能力，这表明类似的光照预处理可改善胁迫条件下的植物性能。
