A Water Solution from the Seeds, Seedlings and Young Plants of the Corn Cockle () Showed Plant-Growth Regulator Efficiency.

Corn cockle ( L. ( (L.) Scop.)) is the main ingredient in some plant preparations for biostimulation in agriculture, and it elicits many positive responses. In our study, we attempted to determine if the fresh and dry plant material of contained auxin-like and cytokinin-like growth regulators (PGRs). and mung bean bioassays were used to determine the presence of auxin-like PGRs and and bioassays were used to determine the presence of cytokinin-like PGRs. A water solution derived from the crushed, homogenized and extracted seeds, fresh and dry seedlings, and fresh and dry young plants showed auxin-like activity in both bioassays. The activity in the bioassay corresponded to 0.5 to 2 mg L of Indole-3-butyric acid (IBA), and in the mung bean bioassay, the activity corresponded to 0.5 to 4 mg L of IBA. While the same water solutions showed weak or no cytokinin-like activity in the cotyledon expansion bioassay, and they showed an activity of approximately 0.5 to 1 mg L of 6-Benzylaminopurine (BAP) in the bioassay. An LC-MS analysis confirmed the presence of free auxins, low levels of or no auxin analogues, a small amount of free cytokinins and a higher level of their cytokinin analogues in the samples, seeds, dry seedlings and young plants of which was likely related to the fine-tuning between the free and analogue forms of the PGRs in the water solutions used in the experiments.