Hou Mengjuan, Kong Hui, Li Jin, Xia Wenwen, Zhu Jianbo
College of Life Science, Shihezi University, Shihezi 832003, China.
Plants (Basel). 2025 Aug 1;14(15):2360. doi: 10.3390/plants14152360.
Low-temperature stress severely limits plant growth and reduces agricultural productivity. Calmodulin-like (CML) proteins are crucial calcium sensors in plant cold responses. Transcriptome analysis of cold-stressed identified seven differentially expressed genes. qRT-PCR confirmed was strongly induced at 4 °C and -2 °C. Bioinformatics analysis showed that encodes a transmembrane protein containing an EF-hand domain. This protein carries a signal peptide and shows the closest phylogenetic relationship to CML3. Its promoter contains ABA, methyl jasmonate (MeJA), and cold-response elements. plants overexpressing showed significantly higher survival rates at -2 °C than wild-type plants. Under freezing stress, -overexpressing lines exhibited reduced malondialdehyde content, relative electrolyte leakage, and ROS accumulation (HO and O), along with increased proline, soluble sugars, soluble proteins, and total antioxidant capacity (T-AOC). elevated the expression of cold-responsive genes and under normal conditions and further upregulated and at 4 °C. Thus, low temperatures induced expression, which was potentially regulated by ABA/MeJA. enhances freezing tolerance by mitigating oxidative damage through boosted T-AOC and osmoprotectant accumulation while activating the CBF-COR signaling pathway. This gene is a novel target for improving crop cold resistance.
低温胁迫严重限制植物生长并降低农业生产力。类钙调蛋白（CML）是植物冷响应中至关重要的钙传感器。对冷胁迫的转录组分析鉴定出7个差异表达基因。qRT-PCR证实该基因在4℃和-2℃时强烈诱导表达。生物信息学分析表明，该基因编码一个含有EF-手型结构域的跨膜蛋白。该蛋白带有信号肽，与CML3的亲缘关系最近。其启动子含有脱落酸（ABA）、茉莉酸甲酯（MeJA）和冷响应元件。过表达该基因的植株在-2℃时的存活率显著高于野生型植株。在冻害胁迫下，过表达该基因的株系丙二醛含量、相对电解质渗漏率和活性氧积累（H₂O₂和O₂⁻）降低，同时脯氨酸、可溶性糖、可溶性蛋白含量和总抗氧化能力（T-AOC）增加。在正常条件下，该基因提高了冷响应基因COR15a和RD29A的表达，在4℃时进一步上调了这两个基因的表达。因此，低温诱导该基因表达，这可能受ABA/MeJA调控。该基因通过增强T-AOC和渗透保护剂积累减轻氧化损伤，同时激活CBF-COR信号通路，从而提高植物的抗冻性。该基因是提高作物抗寒性的一个新靶点。
