Clapa Doina, Hârţa Monica, Radomir Ana Maria, Peticilă Adrian George, Leopold Loredana, Ranga Floricuţa, Sumedrea Dorin Ioan
Faculty of Horticulture and Business in Rural Development, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, Romania.
National Research and Development Institute for Biotechnology in Horticulture Ștefănești-Argeș, 117715 Ștefănești, Romania.
Plants (Basel). 2025 Aug 6;14(15):2437. doi: 10.3390/plants14152437.
This study evaluated biomass accumulation and phenolic compound production in seven species (, , , , , , and ) cultivated in vitro under varying growth regulator treatments and culture periods. Shoots were grown on Murashige and Skoog (MS) medium supplemented with benzyladenine (BA) or meta-topoline (mT) and analyzed after 40 and 60 days. MS medium supplemented with 0.2 mg/L BA was the most effective condition for promoting biomass across all species, with shoot fresh weight increasing significantly at 60 days, particularly in , , and . High-performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS) identified 13 phenolic compounds, including flavonols, hydroxycinnamic acids, anthocyanins, phloroglucinols, and naphthodianthrones. Phenolic profiles were species-specific and influenced by culture period. accumulated the highest total phenolic content (37.6 mg/g DW), while was the top producer of hypericin and pseudohypericin. These results highlight the crucial role of culture conditions in regulating both biomass and phytochemical production and provide a promising approach for producing bioactive metabolites in species through in vitro systems.
本研究评估了在不同生长调节剂处理和培养时期下，七种物种（、、、、、、和）在体外培养时的生物量积累和酚类化合物生成情况。芽苗在添加了苄基腺嘌呤（BA）或间-托布津（mT）的Murashige和Skoog（MS）培养基上生长，并在40天和60天后进行分析。添加0.2 mg/L BA的MS培养基是促进所有物种生物量积累的最有效条件，在60天时芽苗鲜重显著增加，尤其是在、和中。高效液相色谱结合二极管阵列检测和电喷雾电离质谱（HPLC-DAD-ESI-MS）鉴定出13种酚类化合物，包括黄酮醇、羟基肉桂酸、花青素、间苯三酚和萘二蒽酮。酚类物质谱具有物种特异性，并受培养时期影响。积累了最高的总酚含量（37.6 mg/g干重），而金丝桃素和假金丝桃素的产量最高。这些结果突出了培养条件在调节生物量和植物化学物质生成方面的关键作用，并为通过体外系统在物种中生产生物活性代谢物提供了一种有前景的方法。
