Department of Organic and Biochemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran; Research Institute of Bioscience and Biotechnology, University of Tabriz, Tabriz, Iran.
Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran; Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Ecotoxicol Environ Saf. 2021 Feb;209:111841. doi: 10.1016/j.ecoenv.2020.111841. Epub 2020 Dec 30.
We report a new green route for preparing MnO/perlite nanocomposites (NCs) by leaf extract of Hypericum perforatum. Characterization of the physicochemical properties of the MnO/perlite-NCs was performed using XRD, FESEM, EDX, FT-IR, and DLS techniques. Furthermore, their effects on the phytochemical classification and growth parameters of H. perforatum shoot cultures were assessed. According to the FESEM image, the synthesized spherical MnO nanoparticles on the sheet-like structure of nano-perlite were formed, ranging about 20-50 nm. In addition, based on the EDX spectra, the elemental analysis showed the presence of Carbon, Oxygen, Silicon, Aluminum, and Manganese elements in the as-synthesized MnO/perlite-NCs. Biological studies confirmed that nano-perlite and MnO/perlite-NCs were non-toxic to H. perforatum shoot cultures and showed positive effects on plant growth in specific concentrations. Overall, phytochemical classification demonstrated that the terpenoids decreased in the evaluated treatments, while hypericin and pseudohypericin were increased in some treatments (25, 50 and 150 mg/L of nano-perlite) relative to control. Metabolomics results suggested that both nano-perlite and MnO/perlite-NCs can be used as elicitors and new nanofertilizers for generating some secondary metabolites.
我们报告了一种通过贯叶连翘叶提取物制备 MnO/珍珠岩纳米复合材料（NCs）的新绿色路线。采用 XRD、FESEM、EDX、FT-IR 和 DLS 技术对 MnO/珍珠岩-NCs 的物理化学性质进行了表征。此外，还评估了它们对贯叶连翘芽培养物的植物化学分类和生长参数的影响。根据 FESEM 图像，在纳米珍珠岩的片状结构上形成了合成的 MnO 纳米粒子，其尺寸约为 20-50nm。此外，根据 EDX 光谱，元素分析表明在合成的 MnO/珍珠岩-NCs 中存在碳、氧、硅、铝和锰元素。生物研究证实，纳米珍珠岩和 MnO/珍珠岩-NCs 对贯叶连翘芽培养物无毒，并在特定浓度下对植物生长有积极影响。总体而言，植物化学分类表明，在所评估的处理中萜类化合物减少，而在一些处理（25、50 和 150mg/L 的纳米珍珠岩）中，金丝桃素和伪金丝桃素增加。代谢组学结果表明，纳米珍珠岩和 MnO/珍珠岩-NCs 均可作为诱导剂和新型纳米肥料，用于产生一些次生代谢物。
