Rezaie Niloofar, Aghamohammad Shadi, Gholizadeh Khiavi Elham Haj Agha, Pourshafie Mohammad Reza, Talebi Malihe, Rohani Mahdi
Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.
Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
BMC Immunol. 2024 Dec 20;25(1):85. doi: 10.1186/s12865-024-00678-0.
IBD is a condition that may result from the presence of oxidative stress. The objective of this research is to evaluate and compare the potency of probiotics and paraprobiotics to modulate oxidative stress and inflammation.
In the initial phase, the antioxidant capabilities of 88 strains from Lactobacillus and Bifidobacterium were evaluated. In the subsequent phase, during the in-vivo stage, four experimental groups were established, consisting of a high-fat diet (HFD) + PBS, HFD + DSS, HFD + DSS + 10^ cfu/ml of 6 selected native probiotic, and HFD + DSS + 10^ cfu/ml of paraprobiotic (from 6 selected strains), with male wild-type C57BL/6 mice being assigned to these groups. The phenotypical indices and pathological scores along with the evaluation of the expression of genes associated with the NF-kB and Nrf2 signaling pathways, as well as enzymes linked to oxidant/anti-oxidant activities, and proinflammatory/inflammatory cytokines were performed. A significant difference was noted among the groups exposed to DSS and groups that given our native agents. The mice receiving a blend of probiotics and paraprobiotics alongside DSS demonstrated a mitigation of the harmful impacts caused by DSS, both regarding phenotypic traits, including pathological scores and also the level of cytokines and antioxidant markers and also molecular indicators like the Nrf2 and NF-kB associated genes. Also, there was no notable difference between our native probiotic and paraprobiotic.
The study's findings provide evidence that the expression of inflammation can be successfully alleviated by utilizing our native probiotics and paraprobiotics, with a greater emphasis on the latter due to its inherent safety.
This study highlighted the anti-inflammatory and antioxidant properties of probiotic and paraprobiotic that could be useful for patients with inflammatory status.
炎症性肠病（IBD）可能是由氧化应激引起的。本研究的目的是评估和比较益生菌和副益生菌调节氧化应激和炎症的能力。
在初始阶段，评估了88株来自乳酸杆菌和双歧杆菌的抗氧化能力。在随后的阶段，在体内实验阶段，建立了四个实验组，分别为高脂饮食（HFD）+磷酸盐缓冲液（PBS）组、HFD+葡聚糖硫酸钠（DSS）组、HFD+DSS+10^cfu/ml的6种选定的天然益生菌组以及HFD+DSS+10^cfu/ml的副益生菌组（来自6种选定菌株），将雄性野生型C57BL/6小鼠分配到这些组中。进行了表型指标和病理评分，以及与核因子κB（NF-κB）和核因子E2相关因子2（Nrf2）信号通路相关基因的表达评估，以及与氧化/抗氧化活性相关的酶和促炎/炎症细胞因子的评估。在暴露于DSS的组和给予我们的天然制剂的组之间发现了显著差异。同时接受益生菌和副益生菌混合物以及DSS的小鼠在表型特征（包括病理评分）、细胞因子水平和抗氧化标志物以及Nrf2和NF-κB相关基因等分子指标方面，其由DSS造成的有害影响得到了减轻。此外，我们的天然益生菌和副益生菌之间没有显著差异。
该研究结果表明，利用我们的天然益生菌和副益生菌可以成功减轻炎症的表达，由于副益生菌具有内在安全性，因此更强调后者。
本研究突出了益生菌和副益生菌的抗炎和抗氧化特性，这可能对炎症状态的患者有用。
