Ciarochi F F, Robinson A G, Verbalis J G, Seif S M, Zimmerman E A
Peptides. 1985 Sep-Oct;6(5):903-11. doi: 10.1016/0196-9781(85)90321-3.
Neurophysins are part of the prohormones for vasopressin and oxytocin, and are localized with these hormones in the magnocellular cells of the neurohypophysis. New techniques have identified neurophysins in other areas within and outside the central nervous system, and we report here the isolation of neurophysins from the uterus of the rat. Using immunohistology the neurophysin immunoreactivity was localized to the epithelial lining cells of the uterus, and using radioimmunoassay was also present in uterine fluid suggesting secretion into the uterine cavity. The amount of uterine neurophysin increased in response to administered estrogen and was especially elevated in the pregnant uterus. The neurophysin-like material isolated from the uterus was similar to neurophysins from the neurohypophysis by radioimmunoassay, molecular sieve chromatography, isoelectric focusing and SDS gel electrophoresis. Both neurohypophyseal hormones, vasopressin and oxytocin, were also extracted from uterine endothelium and identified by radioimmunoassay and high pressure liquid chromatography.
神经垂体激素运载蛋白是抗利尿激素和催产素前体激素的一部分，与这些激素一起定位于神经垂体的大细胞中。新技术已在中枢神经系统内外的其他区域鉴定出神经垂体激素运载蛋白，我们在此报告从大鼠子宫中分离出神经垂体激素运载蛋白。利用免疫组织学方法，神经垂体激素运载蛋白免疫反应定位于子宫的上皮衬里细胞，利用放射免疫分析法也发现其存在于子宫液中，提示其分泌至子宫腔。给予雌激素后，子宫神经垂体激素运载蛋白的量增加，在妊娠子宫中尤其升高。通过放射免疫分析、分子筛色谱法、等电聚焦和SDS凝胶电泳，从子宫中分离出的神经垂体激素运载蛋白样物质与神经垂体的神经垂体激素运载蛋白相似。抗利尿激素和催产素这两种神经垂体激素也从子宫内皮中提取出来，并通过放射免疫分析和高压液相色谱法进行鉴定。
