Grahovac Marko, Kumric Marko, Vilovic Marino, Supe-Domic Daniela, Pavlovic Nikola, Bukic Josipa, Ticinovic Kurir Tina, Bozic Josko
Department of Endocrinology, Diabetes and Metabolic Diseases, University Hospital of Split, Spinciceva 1, 21000 Split, Croatia.
Department of Pathophysiology, University of Split School of Medicine, Soltanska 2A, 21000 Split, Croatia.
Nutrients. 2025 Jul 31;17(15):2517. doi: 10.3390/nu17152517.
: To determine whether plasma concentrations of anandamide (AEA) and 2-arachidonoylglycerol (2-AG) are elevated in adults with prediabetes, we explored their association with tissue advanced glycation end-products (AGEs) and assessed the influence of Mediterranean diet adherence. : This cross-sectional single-centre study included 92 adults with prediabetes and 86 age-/sex-matched normoglycaemic controls. Anthropometry, blood pressure, biochemical indices, and skin autofluorescence-derived AGEs were measured. Serum AEA and 2-AG were quantified by competitive ELISA, while Mediterranean diet adherence was assessed using the Mediterranean Diet Serving Score (MDSS). : Prediabetes was associated with higher AEA ( = 0.004) but not 2-AG ( = 0.520). Also, AEA correlated positively with AGE values ( = 0.36; = 0.002) and increased across AGE-based cardiovascular risk categories. In multivariable models, both prediabetes status and AGE burden independently predicted AEA. Participants achieving MDSS ≥ 14 exhibited lower AEA ( = 0.038); 2-AG remained unaffected. Finally, the multivariable analysis confirmed that both prediabetes (β = 11.9; = 0.005) and AGE values (β = 0.25; = 0.003) are positively associated with plasma AEA levels, independent of age, sex, BMI, and fasting plasma glucose levels. : Circulating AEA, but not 2-AG, is elevated in prediabetes and independently linked to cumulative AGE burden, suggesting early endocannabinoid activation contributes to cardiometabolic risk. High adherence to a Mediterranean diet may mitigate this dysregulation.
为了确定前驱糖尿病成年人的血浆花生四烯酸乙醇胺（AEA）和2-花生四烯酸甘油酯（2-AG）浓度是否升高，我们探讨了它们与组织晚期糖基化终产物（AGEs）的关联，并评估了坚持地中海饮食的影响。：这项横断面单中心研究纳入了92名前驱糖尿病成年人和86名年龄/性别匹配的血糖正常对照者。测量了人体测量学指标、血压、生化指标以及皮肤自发荧光衍生的AGEs。血清AEA和2-AG通过竞争性酶联免疫吸附测定进行定量，而地中海饮食依从性则使用地中海饮食份数评分（MDSS）进行评估。：前驱糖尿病与较高的AEA相关（P = 0.004），但与2-AG无关（P = 0.520）。此外，AEA与AGE值呈正相关（r = 0.36；P = 0.002），并且在基于AGE的心血管风险类别中升高。在多变量模型中，前驱糖尿病状态和AGE负担均独立预测AEA。MDSS≥14的参与者AEA较低（P = 0.038）；2-AG未受影响。最后，多变量分析证实，前驱糖尿病（β = 11.9；P = 0.005）和AGE值（β = 0.25；P = 0.003）均与血浆AEA水平呈正相关，独立于年龄、性别、体重指数和空腹血糖水平。：前驱糖尿病患者循环中的AEA升高，而2-AG未升高，且AEA与累积AGE负担独立相关，这表明早期内源性大麻素激活会导致心脏代谢风险。高度坚持地中海饮食可能会减轻这种失调。
