Simultaneous Determination of 16 Phenolic Compounds in Edible Fruits from Spontaneous Species Using HPLC-DAD.

A high-performance liquid chromatography with diode array detection (HPLC-DAD) method was optimized and validated for the simultaneous analysis of 16 phenolic compounds, namely gallic acid, protocatechuic acid, -hydroxybenzoic acid, vanillic acid, caffeic acid, catechin, chlorogenic acid, vanillin, syringic acid, coumaric acid, epicatechin, ferulic acid, sinapic acid, salicylic acid, resveratrol, and quercetin, in edible fruits from spontaneous species. Following the validation protocol, the proposed analytical method met the criteria of specificity, linearity, precision, and accuracy. The validated method was then applied for the analysis of phenolic compounds in fruits of hawthorn (), cornelian cherry (), rosehip (), and blueberry (). Of the phenolic compounds investigated, ten were identified in blueberry fruit (), ten in cornelian cherry fruit (), nine in hawthorn fruit (), and seven in rosehip fruit (). In the case of hawthorn (), cornelian cherry (), and blueberry () fruit, epicatechin was identified as the main phenolic compound, while in rosehip () fruit, catechin was the phenolic with the highest content.