Paduch-Cichal Elżbieta, Wakuliński Wojciech, Wilkos Anna, Bączek Katarzyna, Kosakowska Olga, Węglarz Zenon, Mirzwa-Mróz Ewa
Department of Plant Protection, Institute of Horticultural Sciences, Warsaw University of Life Sciences-SGGW (WULS-SGGW), 166 Nowoursynowska Str., 02-787 Warsaw, Poland.
Department of Vegetable and Medicinal Plants, Institute of Horticultural Sciences, Warsaw University of Life Sciences-SGGW (WULS-SGGW), 166 Nowoursynowska Str., 02-787 Warsaw, Poland.
Molecules. 2025 Jul 25;30(15):3122. doi: 10.3390/molecules30153122.
In Poland, the main causal agent of sooty blotch and flyspeck disease is the fungus J.Frank, Schroers et Crous, which is most commonly isolated from the spots found on apples. The aim of the paper was to study the effects of essential oils extracted from Greek oregano, thyme and costmary on . Analysis of the essential oils was conducted using gas chromatography-mass spectrometry (GC-MS) with a flame ionization detector (FID). The Greek oregano essential oil was classified to the carvacrol chemotype, while the thyme and costmary were classified to the thymol and the β-thujone chemotypes, respectively. The influence of these essential oils on the viability of the conidia was analysed cytometrically. The Greek oregano oil was characterised by the significantly highest activity against the spores. The regression analysis performed showed the occurrence of a significant linear relationship between the viability of the conidia and the concentration of the essential oils, which was then the basis for the determination of MICs and MFCs. The values of these parameters in the case of the Greek oregano oil were 0.9 and 0.4%, respectively, and for the thyme oil they were 1.2 and 2.4%.
在波兰，煤烟斑病和蝇粪病的主要致病因子是真菌J.Frank、施勒尔斯和克劳斯，这种真菌最常从苹果上发现的斑点中分离出来。本文的目的是研究从希腊牛至、百里香和艾菊中提取的精油对……的影响。使用带有火焰离子化检测器（FID）的气相色谱-质谱联用仪（GC-MS）对精油进行分析。希腊牛至精油属于香芹酚化学型，而百里香和艾菊精油分别属于百里酚和β-侧柏酮化学型。通过细胞计数法分析了这些精油对分生孢子活力的影响。希腊牛至油对孢子的活性显著最高。进行的回归分析表明，分生孢子的活力与精油浓度之间存在显著的线性关系，这随后成为确定最低抑菌浓度（MICs）和最低杀菌浓度（MFCs）的基础。希腊牛至油的这些参数值分别为0.9%和0.4%，百里香油的这些参数值分别为1.2%和2.4%。
