Amesilide, a New Bicyclic Polyketide from the Marine Fungus MUT6601.

A new bicyclic polyketide, amesilide (), along with the previously reported metabolites, chamisides A (), B (), and E (), chaetoconvosins B () and C (), and chaetochromins A () and B (), were isolated from the marine fungus MUT6601. The structures of the compounds were determined by extensive spectrometric (HRMS) and spectroscopic (1D and 2D NMR) analyses, as well as specific rotation. Absolute configurations of the stereogenic centers of amesilide () were determined by a comparison of its experimental circular dichroism (CD) spectrum with its time-dependent density functional theory (TD-DFT) electronic circular dichroism (ECD) spectra. Among them, chaetochromins A () and B () showed strong antibacterial activity against S25 (MBC values of 12.50 µM and MIC values of 6.25 µM) and a moderate cytotoxicity against monocytes (THP-1) and peripheral blood cells (PBMC) (IC values of 33.65-40.01 µM).