Belmonte-Izquierdo Yadira, Salomé-Abarca Luis Francisco, López Mercedes G, González-Hernández Juan Carlos
Tecnológico Nacional de México/Instituto Tecnológico de Morelia, Av. Tecnológico #1500, Morelia 58120, Mexico.
Colegio de Postgraduados, Km. 36.5 Carretera Federal México-Texcoco Montecillo, Texcoco de Mora 56264, Mexico.
Foods. 2025 Aug 1;14(15):2714. doi: 10.3390/foods14152714.
Fructooligosaccharides (FOS) are short fructans with different degrees of polymerization (DP) and bonds in their structure, generated by the distinct activities of fructosyltransferase enzymes, which produce distinct types of links. FOS are in high demand on the market, mainly because of their prebiotic effects. In recent years, depending on the link type in the FOS structure, prebiotic activity has been shown to be increased. Studies on β-fructanofuranosidases (Ffasa), enzymes with fructosyltransferase activity in yeasts, have reported the production of F-FOS, F-FOS, and G-FOS. The aims of this investigation were to evaluate the capability of fifteen different yeasts to grow in sp. juices and to determine the potential of these juices as substrates for FOS production. Additionally, the research aimed to corroborate and analyze the fructosyltransferase activity of enzymatic extracts obtained from agave yeasts by distinct induction media and to identify the role and optimal parameters (time and sucrose and glucose concentrations) for FOS and disaccharides production through Box-Behnken designs. To carry out such a task, different techniques were employed: FT-IR, TLC, and HPAEC-PAD. We found two yeasts with fructosyltransferase activity, ITMLB97 and ITMLB85. In addition, within the most relevant results, the production of the FOS 1-kestose, 6-kestose, and neokestose, as well as disaccharides inulobiose, levanobiose, and blastose, molecules with potential applications, was determined. Overall, FOS production requires suitable yeast species, which grow in a medium under optimal conditions, from which microbial enzymes with industrial potential can be obtained.
低聚果糖（FOS）是具有不同聚合度（DP）和结构中键的短链果聚糖，由果糖基转移酶的不同活性产生，这些酶产生不同类型的连接。FOS在市场上需求量很大，主要是因为它们的益生元作用。近年来，根据FOS结构中的连接类型，已显示益生元活性有所提高。对β-呋喃果糖苷酶（Ffasa）（酵母中具有果糖基转移酶活性的酶）的研究报告了F-FOS、F-FOS和G-FOS的产生。本研究的目的是评估15种不同酵母在龙舌兰汁中的生长能力，并确定这些汁液作为FOS生产底物的潜力。此外，该研究旨在证实和分析通过不同诱导培养基从龙舌兰酵母中获得的酶提取物的果糖基转移酶活性，并通过Box-Behnken设计确定FOS和二糖生产的作用和最佳参数（时间以及蔗糖和葡萄糖浓度）。为了完成这项任务，采用了不同的技术：傅里叶变换红外光谱（FT-IR）、薄层色谱（TLC）和高效阴离子交换色谱-脉冲安培检测法（HPAEC-PAD）。我们发现了两种具有果糖基转移酶活性的酵母，ITMLB97和ITMLB85。此外，在最相关的结果中，确定了FOS 1-蔗果三糖、6-蔗果三糖和新蔗果三糖以及二糖菊粉二糖、左聚糖二糖和蜜三糖的产生，这些分子具有潜在的应用价值。总体而言，FOS生产需要合适的酵母菌种，它们在最佳条件下的培养基中生长，从中可以获得具有工业潜力的微生物酶。
