Wu Yue, Zhang Yutao, Guo Zhiqian, Xu Yisheng
Center of Photosensitive Chemicals Engineering, State Key Laboratory of Green Chemical Engineering and Industrial Catalysis, State Key Laboratory of Chemical Engineering, School of Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China.
Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
Polymers (Basel). 2025 Aug 6;17(15):2152. doi: 10.3390/polym17152152.
Fluorescent dyes, such as cyanine dyes, are widely used in fluorescence-imaging-guided tumor therapy due to their high absorbance and fluorescence quantum yield. However, challenges persist in optimizing the performance of fluorescent nanoparticles, particularly due to the aggregation-caused quenching (ACQ) effect of cyanine dyes. Here, a novel counterion construction strategy is introduced using cyanine dye as a model ACQ dye. Through dynamic-controlled flash nanoprecipitation, fluorescent nanoparticles (CyINPs) with tunable structures are developed, investigating the effects of various factors, including counterions, block copolymers, and dye concentrations, on CyINPs' stability and fluorescence enhancement. The optimized CyINPs with good water solubility show a 21-fold increase in fluorescence intensity and a 3.5-fold increase in encapsulation efficiency compared to CyINPs prepared by a thermodynamic-driven method. Under the efforts of polymers and counterions, dyes are separated, which reduces the impact of the ACQ effect and results in stronger fluorescence intensity, providing insights into improving nanoparticle biocompatibility and energy utilization efficiency.
荧光染料，如菁染料，由于其高吸光度和荧光量子产率，在荧光成像引导的肿瘤治疗中被广泛应用。然而，在优化荧光纳米颗粒的性能方面仍然存在挑战，特别是由于菁染料的聚集诱导猝灭（ACQ）效应。在此，以菁染料作为典型的ACQ染料，引入了一种新型抗衡离子构建策略。通过动态控制的快速纳米沉淀法，制备了结构可调的荧光纳米颗粒（CyINPs），研究了包括抗衡离子、嵌段共聚物和染料浓度等各种因素对CyINPs稳定性和荧光增强的影响。与通过热力学驱动方法制备的CyINPs相比，优化后的具有良好水溶性的CyINPs荧光强度提高了21倍，包封率提高了3.5倍。在聚合物和抗衡离子的作用下，染料得以分离，这减少了ACQ效应的影响，导致荧光强度增强，为提高纳米颗粒的生物相容性和能量利用效率提供了思路。
