Kincade P W, Ralph P
Cold Spring Harb Symp Quant Biol. 1977;41 Pt 1:245-50. doi: 10.1101/sqb.1977.041.01.030.
Regulatory effects of antibodies to cell-surface antigens have been examined in an in vitro clonal assay for B lymphocytes. In this system, cell contact is prevented by a semisolid gel matrix, and many of the factors affecting cell proliferation have been described. Inclusion of antibodies to mu, kappa, or Ia in these cultures reduced the size and number of proliferating foci. As little as 1 microgram/ml of anti-mu antibody prevented formation of colony-size aggregates. Pretreatment with these antibodies before culture followed by wash had no effect. Divalent F(ab')2 anti-IgM antibodies were as effective as intact antibodies, but monovalent Fab fragments were not inhibitory. Anti-gamma1 antibodies, anti-alpha antibodies, or cell-bound antigen-antibody complexes had no effect on clonal proliferation. These findings suggest that a negative signal can be delivered to B cells during a critical period of their activation via certain surface receptors. Modifications in culture conditions result in the selective propagation of different B-cell subpopulations.
已在B淋巴细胞的体外克隆试验中研究了针对细胞表面抗原的抗体的调节作用。在该系统中，半固体凝胶基质可防止细胞接触，并且已描述了许多影响细胞增殖的因素。在这些培养物中加入针对μ、κ或Ia的抗体可减少增殖灶的大小和数量。低至1微克/毫升的抗μ抗体可阻止形成集落大小的聚集体。培养前用这些抗体预处理然后洗涤没有效果。二价F(ab')2抗IgM抗体与完整抗体一样有效，但单价Fab片段没有抑制作用。抗γ1抗体、抗α抗体或细胞结合的抗原-抗体复合物对克隆增殖没有影响。这些发现表明，在B细胞激活的关键时期，可通过某些表面受体向其传递负信号。培养条件的改变导致不同B细胞亚群的选择性增殖。
