Abou-El-Naga Amoura M, Abdraboh Mohamed E, El-Magd Mohammed A, Mansour Sameh, Mansour Hend Abd El-Halim
Zoology Department, Faculty of Science, Mansoura University, Mansoura, Egypt.
Anatomy Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Shaikh, Egypt.
Sci Rep. 2025 Aug 17;15(1):30072. doi: 10.1038/s41598-025-15456-0.
Semen cryopreservation is a crucial technique for preserving male fertility, playing a vital role in assisted reproductive procedures by storing frozen semen samples for artificial insemination (AI) and intra-cytoplasmic sperm injection (ICSI) to enhance reproductive success rates. This study aims to identify the most effective cryopreservation methods and assess their impact on semen quality, particularly sperm DNA fragmentation. A total of 30 semen samples were categorized into fertile and infertile groups. DNA fragmentation analysis was conducted using the Sperm Chromatin Structure Assay (SCSA). Each sample was divided into three portions and frozen using different cryoprotectants: (egg-yolk + glycerol), (sucrose + glycerol), and (glycerol alone). After one month of storage, the samples were analyzed to determine the most effective medium. The findings revealed a decline in sperm motility post-freezing compared to fresh samples, along with a slight increase in morphological abnormalities. Additionally, there was a rise in sperm DNA fragmentation and an increase in apoptotic marker (Caspase-3) levels after the freezing process. The study concluded that cryopreservation and thawing caused some degree of sperm cell damage, with infertile samples being more adversely affected than fertile ones.
精液冷冻保存是一项保存男性生育能力的关键技术，通过储存冷冻精液样本用于人工授精（AI）和卵胞浆内单精子注射（ICSI）以提高生殖成功率，在辅助生殖程序中发挥着至关重要的作用。本研究旨在确定最有效的冷冻保存方法，并评估其对精液质量的影响，特别是对精子DNA片段化的影响。总共30份精液样本被分为可育组和不育组。使用精子染色质结构分析（SCSA）进行DNA片段化分析。每个样本被分成三份，并使用不同的冷冻保护剂进行冷冻：（蛋黄+甘油）、（蔗糖+甘油）和（单独甘油）。储存一个月后，对样本进行分析以确定最有效的培养基。研究结果显示，与新鲜样本相比，冷冻后精子活力下降，形态异常略有增加。此外，冷冻过程后精子DNA片段化增加，凋亡标志物（半胱天冬酶-3）水平升高。该研究得出结论，冷冻保存和解冻会导致一定程度的精子细胞损伤，不育样本比可育样本受到的负面影响更大。
