Sahudin Muhammad Ameerullah, Tan Jian Xiong, Azmi Khairun Nasriah, Suhaimi Nur Afny, Hisham Shameer, Abu Bakar Nor Kartini, Zainol Abidin Muhammad Nidzhom, Ishak Khairul Anwar, Abd Karim Nurul Huda
Department of Chemistry, Faculty of Science, Universiti Malaya, Kuala Lumpur, 50603, Malaysia.
Institute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi, 43600, Selangor, Malaysia.
J Fluoresc. 2025 Aug 16. doi: 10.1007/s10895-025-04486-6.
Biogenic amines serve as valuable indicators within the food industry, as they can identify signs of food spoilage. Excessive intake of these amines can potentially result in scombroid poisoning, headaches, respiratory issues, and other health-related issues. In recent times, more streamlined and rapid techniques for detecting biogenic amines have emerged, utilizing metal complex as optical sensing materials. This research focused on the development of putrescine fluorescence sensor based on PMMA-zinc(II) Schiff base complex. In the first stage, zinc(II) N,N'-bis[4-(hydroxysalicylidene)] phenylenediamine, complex 1 was synthesized and characterized using spectroscopic techniques. The second stage of this work involved studying the interaction between the complex with putrescine using UV-Vis titration and fluorescence emission titration. UV-Vis titration findings indicate that the complex was able to interact with putrescine strongly with binding constant, K = (2.92 ± 0.45) × 10 M. Optical putrescine sensor with zinc(II) complex deposited onto polymethyl methacrylate (PMMA) nanoparticles as immobilization support was fabricated and successfully responded to putrescine. Under ideal conditions, the developed sensor can detect down to 3.59 × 10 M in the range of between 1.0 × 10 M and 1.0 × 10 M (R = 0.9884). Selectivity performance of the sensor towards putrescine over other amines was confirmed. Additionally, the sensor exhibited excellent reproducibility, as indicated by low relative standard deviation values. Furthermore, the proposed sensor has been effectively utilized to detect putrescine in buffalo meat, yielding satisfactory recovery rates. This method holds significant promise in the food quality assurance, particularly in improving food safety to ensure healthy consumption.
生物胺在食品工业中是有价值的指标，因为它们可以识别食品变质的迹象。过量摄入这些胺可能会导致组胺中毒、头痛、呼吸问题以及其他与健康相关的问题。近年来，出现了更简化、快速的生物胺检测技术，利用金属配合物作为光学传感材料。本研究聚焦于基于聚甲基丙烯酸甲酯 - 锌（II）席夫碱配合物的腐胺荧光传感器的开发。在第一阶段，合成了锌（II）N,N'-双[4 -（羟基水杨醛）]苯二胺配合物1，并使用光谱技术对其进行了表征。这项工作的第二阶段涉及使用紫外 - 可见滴定和荧光发射滴定研究该配合物与腐胺之间的相互作用。紫外 - 可见滴定结果表明，该配合物能够与腐胺强烈相互作用，结合常数K =（2.92 ± 0.45）× 10 M。制备了将锌（II）配合物沉积在聚甲基丙烯酸甲酯（PMMA）纳米颗粒上作为固定化载体的光学腐胺传感器，该传感器对腐胺成功做出了响应。在理想条件下，所开发的传感器在1.0 × 10 M至1.0 × 10 M的范围内能够检测低至3.59 × 10 M的腐胺（R = 0.9884）。证实了该传感器对腐胺相对于其他胺的选择性性能。此外，如低相对标准偏差值所示，该传感器表现出优异 的重现性。此外，所提出的传感器已有效地用于检测水牛肉中的腐胺，回收率令人满意。该方法在食品质量保证方面具有重大前景，特别是在提高食品安全以确保健康消费方面。
