Gleeson M J
Acta Otolaryngol. 1985 Nov-Dec;100(5-6):419-28. doi: 10.3109/00016488509126566.
The use of SEM as an adjunct to TEM and electrophysiological examination of the cochlea is now well established. It has provided a relatively simple method of assessing the effect of noise, ototoxic drugs, electrical stimulation, etc. on the surface features of the organ of corti. A controlled experiment was undertaken to document the surface autolytic changes in rat cochleas by fixation at intervals up to eight hours post mortem. These were compared with human material fixed between 40 minutes and eight hours post mortem. The results complement previous light and transmission electron microscopy studies, gives insight into the optimal and acceptable fixation times for the two species and act as a guide for the interpretation of human material.
扫描电子显微镜（SEM）作为透射电子显微镜（TEM）和耳蜗电生理检查的辅助手段，其应用现已得到充分确立。它提供了一种相对简单的方法，用于评估噪声、耳毒性药物、电刺激等对柯蒂氏器表面特征的影响。我们进行了一项对照实验，通过在死后长达八小时的不同时间间隔进行固定，记录大鼠耳蜗的表面自溶变化。并将这些结果与死后40分钟至八小时之间固定的人体材料进行比较。这些结果补充了先前的光学和透射电子显微镜研究，深入了解了这两个物种的最佳和可接受的固定时间，并为解释人体材料提供了指导。
