Liu Wei, Jiang Yingyan, Wang Cheng, Wang Min, Zhang Wei, Ren Haiying, Xu Shiyan, Qin Jinjing, Liu Pan, Jin Lianhai, Zhao Donghai
College of Laboratory Medicine, Jilin Medical University, Jilin, China.
Department of Obstetrics and Gynecology, Affiliated hospital of Jilin medical university, Jilin, China.
Front Cell Infect Microbiol. 2025 Aug 4;15:1627668. doi: 10.3389/fcimb.2025.1627668. eCollection 2025.
HPV has been conclusively associated with various human malignancies, making the development of prevention and treatment strategies for HPV-induced diseases a high priority. Currently, primary prevention methods include HPV immunization and routine screening, which significantly reduce the risk of HPV transmission. However, for patients diagnosed with invasive, advanced, or recurrent malignancies, non-virus-specific therapies frequently lead to drug resistance and adverse effects, resulting in minimal improvement in treatment efficacy for numerous patients. Viral genome-targeting therapy is emerging as a promising avenue for the future management of HPV infections. With the rapid advancement of genetic modification technologies, the CRISPR/Cas system has demonstrated significant potential in treating viral infections. Its ability to selectively target and edit viral genomes for elimination positions it as a highly effective approach for combating HPV. This review will explore the functions and applications of the CRISPR/Cas system as an innovative therapy for HPV. We will illustrate the prospective efficacy of CRISPR/Cas as a groundbreaking and promising cure for HPV infections, while also addressing the opportunities and challenges associated with this novel approach.
人乳头瘤病毒（HPV）已被确凿地证明与多种人类恶性肿瘤相关，这使得开发针对HPV引发疾病的预防和治疗策略成为当务之急。目前，一级预防方法包括HPV免疫接种和常规筛查，这些方法能显著降低HPV传播风险。然而，对于被诊断为侵袭性、晚期或复发性恶性肿瘤的患者，非病毒特异性疗法常常导致耐药性和不良反应，致使众多患者的治疗效果改善甚微。靶向病毒基因组的疗法正成为未来管理HPV感染的一条有前景的途径。随着基因编辑技术的迅速发展，CRISPR/Cas系统在治疗病毒感染方面已展现出巨大潜力。它能够选择性地靶向和编辑病毒基因组以实现清除，这使其成为对抗HPV的一种高效方法。本综述将探讨CRISPR/Cas系统作为一种针对HPV的创新疗法的功能及应用。我们将阐述CRISPR/Cas作为一种开创性且有前景的HPV感染治疗方法的预期疗效，同时也会探讨与这种新方法相关的机遇和挑战。
