Cold stress enhances cryotolerance in B6 via membrane lipid remodeling and differential protein expression.

Understanding the molecular mechanisms underlying the cryotolerance of lactic acid bacteria is critical for preserving their viability in food processing. In this study, B6 and KF7 with different phenotypic properties were pretreated at 4 °C for 2 h before liquid nitrogen freezing. Cold-stressed B6 exhibited significantly higher survival (53 %) than KF7 (30 %) and untreated controls (44 % vs. 10 %, < 0.05), with few disrupted cells observed under SEM. Cold stress altered the membrane fluidity in B6 by increasing unsaturated fatty acids (UFA) and the ratio of UFA to saturated fatty acids (UFA/SFA) from 1.36 to 1.62 ( < 0.05). 219 proteins, primarily those involved in fatty acid biosynthesis, translation and transport processes were up regulated in cold-stressed B6, compared to untreated counterparts. Noteworthy, capsules of B6 likely contributed to its higher cryotolerance. Our findings revealed a dual cryotolerance mechanism in L. B6, i.e., dynamic lipidome remodeling and coordinated protein expression regulation. This study highlighted the importance of the cell surface features of individual strain of L. and cold stress treatment in preparing probiotics as well as direct to vat starter cultures with high cell vitality employing deep freezing process.