Hochenegger Nadine J, Vignolle Gabriel A, Schmal Matthias, Mach Robert L, Mach-Aigner Astrid R, Rahimi Mohammad Javad, Chan Chin Mei, Cai Feng M, Druzhinina Irina S, Zimmermann Christian
Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Gumpendorfer Strasse 1a, Wien, 1060, Austria.
The Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
BMC Microbiol. 2025 Aug 21;25(1):526. doi: 10.1186/s12866-025-04274-z.
Fungi play essential ecological roles and have been utilized by humans for diverse applications such as industrial enzyme production or as sources of bioactive compounds. Recent research has highlighted the Amphisphaeriales order (Ascomycota) as promising producers of secondary metabolites of pharmaceutical importance. Within this family, the recently established genus Neoarthrinium includes species such as N. brasiliense, N. lithocarpicola, N. moseri, N. trachycarpi, and N. urticae. Existing literature has primarily focused on the taxonomy and phylogeny of Neoarthrinium, leaving its physiology, ecology, and metabolic potential unexplored.
This study presents the first investigation of the metabolic and genomic potential of N. moseri. We describe the isolation of two South-Asian N. moseri strains and the genome sequencing of these strains alongside the Colombian ex-type strain for the species. Comparative genome analysis reveals an exceptionally high number of biosynthetic gene clusters (BGCs), surpassing those of many other fungi in the Amphisphaeriales order. Additionally, the genome of N. moseri contains a diverse repertoire of carbohydrate-active enzymes (CAZymes), supporting its hypothesized ecological role as a phyllosphere fungus (putatively an endophyte and/or saprotroph). Ecophysiological assays, including BIOLOG phenotyping, demonstrate its ability to utilize a broad range of carbon sources, emphasizing ecological versatility.
This study highlights N. moseri as a promising candidate for secondary metabolite discovery, providing foundational insights into the metabolic and genomic potential of the Neoarthrinium genus and related fungi.
真菌在生态系统中发挥着重要作用，并且已被人类用于多种用途，如工业酶生产或作为生物活性化合物的来源。最近的研究强调了格孢腔菌目（子囊菌门）是具有重要药用价值的次生代谢产物的有前景的生产者。在这个科中，最近建立的新节球壳属包括巴西新节球壳、石栎新节球壳、莫泽新节球壳、棕榈新节球壳和荨麻新节球壳等物种。现有文献主要集中在新节球壳属的分类学和系统发育方面，而其生理学、生态学和代谢潜力尚未得到探索。
本研究首次对莫泽新节球壳的代谢和基因组潜力进行了调查。我们描述了从南亚分离出的两株莫泽新节球壳菌株，并对这些菌株以及该物种的哥伦比亚模式菌株进行了基因组测序。比较基因组分析显示，其生物合成基因簇（BGCs）数量异常之多，超过了格孢腔菌目中的许多其他真菌。此外，莫泽新节球壳的基因组包含多种碳水化合物活性酶（CAZymes），支持了其作为叶际真菌（可能是内生菌和/或腐生菌）的假定生态作用。包括BIOLOG表型分析在内的生态生理学测定表明，它能够利用多种碳源，突出了其生态多功能性。
本研究强调莫泽新节球壳是次生代谢产物发现的一个有前景的候选者，为新节球壳属及相关真菌的代谢和基因组潜力提供了基础见解。
