Hameed Mamoona, Javed Saman, Arshad Najma, Gul Zaman, Mushtaq Maria, Munir Aneela, Shazadi Kiran, Arshad Muhammad, Noureen Saleha
Institute of Zoology, University of the Punjab, Lahore, Pakistan.
Department of Biology, Lahore Garrison University, Lahore, Pakistan.
Curr Microbiol. 2025 Aug 22;82(10):469. doi: 10.1007/s00284-025-04382-z.
Heavy metal contamination, particularly cadmium (Cd), poses serious health risks through bioaccumulation and oxidative stress. Certain lactic acid bacteria (LAB) exhibit metal-binding properties and have demonstrated potential in mitigating Cd toxicity. This study evaluated the ameliorative effects of two locally isolated LAB strains Levilactobacillus brevis MW362790 and Pediococcus pentosaceus MT323062 against Cd-induced toxicity in rat model. Over 12 weeks of experiment, rats were divided into four groups (I-IV): a negative control, a CdCl₂-intoxicated positive control (3 mg/kg body weight), and two treatment groups receiving CdCl₂ along with either L. brevis or P. pentosaceus (5 × 10⁹ CFU/kg body weight), respectively. Blood samples were collected fortnightly, while liver, kidney, adipose tissue, and fecal samples were collected at the end of the experiment. Compared to the positive control, both probiotic-treated groups (III and IV) displayed significantly reduced Cd accumulation in tissues, improved levels of hepatic and renal function markers, decreased malondialdehyde and total oxidative stress markers, and enhanced enzymatic antioxidant activities. Histopathological evaluations further corroborated these findings, by revealing reduced tissue damage in treated animals. Moreover, the increased fecal Cd excretion in the same groups indicated effective intestinal Cd elimination. This is the first report demonstrating Cd-attenuating potential of L. brevis MW362790, underscoring the therapeutic significance of locally isolated LAB strains in managing heavy metal toxicity.
重金属污染，尤其是镉（Cd），通过生物累积和氧化应激带来严重的健康风险。某些乳酸菌（LAB）具有金属结合特性，并已证明在减轻镉毒性方面具有潜力。本研究评估了两种本地分离的LAB菌株短乳杆菌MW362790和戊糖片球菌MT323062对大鼠模型中镉诱导毒性的改善作用。在为期12周的实验中，大鼠被分为四组（I-IV）：一个阴性对照组、一个CdCl₂中毒阳性对照组（3毫克/千克体重）以及两个分别接受CdCl₂与短乳杆菌或戊糖片球菌（5×10⁹CFU/千克体重）联合处理的治疗组。每两周采集一次血样，在实验结束时采集肝脏、肾脏、脂肪组织和粪便样本。与阳性对照组相比，两个益生菌处理组（III和IV）在组织中的镉积累均显著减少，肝肾功能标志物水平得到改善，丙二醛和总氧化应激标志物减少，酶促抗氧化活性增强。组织病理学评估通过揭示处理动物中组织损伤的减轻进一步证实了这些发现。此外，同一组中粪便镉排泄增加表明肠道对镉的有效清除。这是首次报道短乳杆菌MW362790具有减轻镉毒性的潜力，强调了本地分离的LAB菌株在管理重金属毒性方面的治疗意义。
