Azmy Eman, El-Zehary Rehab R A, Ibrahim Fatma M M, Denewar Mona
University Students Hospital, Mansoura University, Mansoura City, Egypt.
Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura City, Egypt.
Oral Maxillofac Surg. 2025 Aug 23;29(1):147. doi: 10.1007/s10006-025-01433-4.
The tongue plays a vital role in physiological functions where tissue loss impairs the patient's life, so regenerative approaches are crucial. This study was performed to explore the synergetic possibility of gold nanoparticles (AuNPs) and bone marrow stem cells (BMSCs) in tongue defect regeneration.
Rats were subjected to surgical defects on the tongue's dorsal surface and divided into four groups: Group I (control): received no treatment, Group II (AuNPs): received 0.1 ml AuNPs solution (50 nm diameter) 1 mm sub-epithelial, Group III (BMSCs): received 0.1 ml BMSCs (3.5 × 105 cells/cm2), and Group IV (BMSCs/AuNPs) received 0.1 ml BMSCs (3.5 × 105 cells/cm2) previously cultured with 6 µg/ml AuNPs. Circumferential injections were made around the defect. Samples were collected and prepared for histological and immunohistochemical staining four and seven days after treatment, and then histomorphometric and statistical analyses were performed.
The BMSCs/AuNPs group revealed better histological regeneration, including consistent epithelial stratification with a thick keratin layer, a regularly arranged basal cell layer, more vascularity, and well-arranged muscle bundles in connective tissue with fewer inflammatory cells than other groups. Regarding immunohistochemical. results, the BMSCs/AuNPs group demonstrated a significant increase in PCNA and S100 expression in both time intervals in comparison with other groups.
Wound healing after AuNPs in conjugation with BMSCs emphasized their synergetic effect on BMSCs. Attained. results extend to applied nanotechnology and regenerative medicine, supporting the application of AuNPs in wound healing fields.
舌头在生理功能中起着至关重要的作用，组织缺失会损害患者的生活，因此再生方法至关重要。本研究旨在探索金纳米颗粒（AuNPs）与骨髓干细胞（BMSCs）在舌缺损再生中的协同可能性。
对大鼠舌背表面进行手术缺损，并分为四组：第一组（对照组）：不接受治疗；第二组（AuNPs组）：在黏膜下1mm处接受0.1ml AuNPs溶液（直径50nm）；第三组（BMSCs组）：接受0.1ml BMSCs（3.5×105个细胞/cm2）；第四组（BMSCs/AuNPs组）接受0.1ml先前与6μg/ml AuNPs共同培养的BMSCs（3.5×105个细胞/cm2）。在缺损周围进行环形注射。治疗后第4天和第7天收集样本并制备用于组织学和免疫组织化学染色，然后进行组织形态计量学和统计分析。
BMSCs/AuNPs组显示出更好的组织学再生，包括一致的上皮分层，角质层增厚，基底细胞层排列规则，血管更丰富，结缔组织中肌束排列良好，炎症细胞比其他组少。关于免疫组织化学结果，BMSCs/AuNPs组在两个时间间隔内PCNA和S100表达均较其他组显著增加。
AuNPs与BMSCs结合后的伤口愈合强调了它们对BMSCs的协同作用。所得结果扩展到应用纳米技术和再生医学，支持AuNPs在伤口愈合领域的应用。
