文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

单分散和混合尺寸金纳米粒子在侧向流动分析检测白蛋白中的比较。

Comparison of Single- and Mixed-Sized Gold Nanoparticles on Lateral Flow Assay for Albumin Detection.

机构信息

Materials Science and Engineering Program, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.

Nanobiotechnology and Nanobiomaterials Research Laboratory, School of Materials Science and Innovation, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.

出版信息

Biosensors (Basel). 2021 Jun 26;11(7):209. doi: 10.3390/bios11070209.

DOI:10.3390/bios11070209
PMID:34206883
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8301757/
Abstract

The sensitivity and reproducibility of the lateral flow assay can be influenced by multiple factors, such as the size of gold nanoparticles (GNPs) employed. Here, we evaluated the analytical performance of single-sized and mixed-sized GNPs using a simple lateral flow assay (LFA) platform. This platform was used as a model assay to diagnose albumin levels and demonstrate the analytical performance of single-sized and mixed-sized GNPs in LFA tests. Two sizes of GNPs@anti-bovine serum albumin (BSA) conjugate proteins were mixed at different ratios. The unique optical properties of the GNPs induced a distinguishing color-shedding effect on the single- and mixed-sized GNPs@anti-BSA conjugates interacting with the target analyte BSA spotted on the test line. The use of mixed-sized GNPs@anti-BSA conjugates enhanced signal relative to the 20 nm GNPs, and provided superior stability compared with solely employing the large GNPs (50 nm). The proposed platform in this study could provide an efficient BSA detection mechanism that can be utilized as a model biomarker for confronting chronic kidney disease.

摘要

金纳米颗粒（GNPs）的尺寸等多种因素会影响侧向流动检测法的灵敏度和重现性。在此，我们使用简单的侧向流动检测法（LFA）平台评估了单分散和混合尺寸 GNPs 的分析性能。该平台被用作模型检测法，用于诊断白蛋白水平，并展示单分散和混合尺寸 GNPs 在 LFA 检测中的分析性能。两种尺寸的 GNPs@抗牛血清白蛋白（BSA）缀合物蛋白以不同比例混合。GNPs 的独特光学特性在单分散和混合尺寸 GNPs@抗 BSA 缀合物与检测线上点样的靶标分析物 BSA 相互作用时引起了明显的颜色褪去效应。与使用 20nm GNPs 相比，混合尺寸 GNPs@抗 BSA 缀合物增强了信号，并且与仅使用大尺寸 GNPs（50nm）相比，提供了更好的稳定性。本研究中提出的平台可以提供一种有效的 BSA 检测机制，可用作应对慢性肾病的模型生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31ed/8301757/7308f6f560f4/biosensors-11-00209-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31ed/8301757/78ab9ae9a6b0/biosensors-11-00209-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31ed/8301757/32b58e2e24ee/biosensors-11-00209-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31ed/8301757/aa3f7f1fd9f3/biosensors-11-00209-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31ed/8301757/cdc2bf7662ad/biosensors-11-00209-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31ed/8301757/af8c10e8433e/biosensors-11-00209-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31ed/8301757/ab9faaf6a7bc/biosensors-11-00209-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31ed/8301757/0b3f9ff70860/biosensors-11-00209-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31ed/8301757/7308f6f560f4/biosensors-11-00209-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31ed/8301757/78ab9ae9a6b0/biosensors-11-00209-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31ed/8301757/32b58e2e24ee/biosensors-11-00209-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31ed/8301757/aa3f7f1fd9f3/biosensors-11-00209-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31ed/8301757/cdc2bf7662ad/biosensors-11-00209-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31ed/8301757/af8c10e8433e/biosensors-11-00209-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31ed/8301757/ab9faaf6a7bc/biosensors-11-00209-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31ed/8301757/0b3f9ff70860/biosensors-11-00209-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31ed/8301757/7308f6f560f4/biosensors-11-00209-g008.jpg

相似文献

[1]
Comparison of Single- and Mixed-Sized Gold Nanoparticles on Lateral Flow Assay for Albumin Detection.

Biosensors (Basel). 2021-6-26

[2]
Nanoparticle-protein interactions: a thermodynamic and kinetic study of the adsorption of bovine serum albumin to gold nanoparticle surfaces.

Langmuir. 2013-11-20

[3]
Inhibitory effect of gold nanoparticles on the D-ribose glycation of bovine serum albumin.

Int J Nanomedicine. 2014-11-26

[4]
Morphological effect of gold nanoparticles on the adsorption of bovine serum albumin.

Phys Chem Chem Phys. 2014-10-14

[5]
Size and macromolecule stabilizer-dependent performance of gold colloids in immuno-PCR.

Anal Bioanal Chem. 2022-3

[6]
Complex analysis of concentrated antibody-gold nanoparticle conjugates' mixtures using asymmetric flow field-flow fractionation.

J Chromatogr A. 2016-12-16

[7]
Radiosensitizing effects of different size bovine serum albumin-templated gold nanoparticles on H22 hepatoma-bearing mice.

Nanomedicine (Lond). 2018-5-11

[8]
Quantitative biokinetics and systemic translocation of various gold nanostructures are highly dependent on their size and shape.

J Nanosci Nanotechnol. 2014-6

[9]
Nepenthes khasiana mediated synthesis of stabilized gold nanoparticles: Characterization and biocompatibility studies.

J Photochem Photobiol B. 2016-1

[10]
Adsorption and conformation of serum albumin protein on gold nanoparticles investigated using dimensional measurements and in situ spectroscopic methods.

Langmuir. 2011-2-22

引用本文的文献

[1]
The synergetic potential of gold nanoparticles and bone marrow stem cells in regeneration of tongue defects (An experimental study on Rats).

Oral Maxillofac Surg. 2025-8-23

[2]
Current and future opportunities for lateral flow immunoassay strips based on tunable plasmonic properties of gold nanoparticles.

Mikrochim Acta. 2025-7-12

[3]
Development and characterization of gold nanoparticle-based colorimetric sensing assay functionalized with L-cysteine for the detection of calcium carbide in ripened mango and banana fruits.

Heliyon. 2025-2-15

[4]
Gold nanoparticles coated with collagen-I and their wound healing activity in human skin fibroblast cells.

Heliyon. 2024-6-20

[5]
Recent Update Roles of Magnetic Nanoparticles in Circulating Tumor Cell (CTC)/Non-CTC Separation.

Pharmaceutics. 2023-10-17

[6]
Nanoarchitectonics of photothermal materials to enhance the sensitivity of lateral flow assays.

Beilstein J Nanotechnol. 2023-10-4

[7]
Development of a Biotinylated Nanobody-Based Gold Nanoparticle Immunochromatographic Assay for the Detection of Procymidone in Crops.

J Agric Food Chem. 2023-9-6

本文引用的文献

[1]
Highly Sensitive Chemiluminescence-Based Lateral Flow Immunoassay for Cardiac Troponin I Detection in Human Serum.

Sensors (Basel). 2020-5-2

[2]
A network composed of gold nanoparticles and a poly(vinyl alcohol) hydrogel for colorimetric determination of ceftriaxone.

Mikrochim Acta. 2020-1-15

[3]
Revealing the Importance of Aging, Environment, Size and Stabilization Mechanisms on the Stability of Metal Nanoparticles: A Case Study for Silver Nanoparticles in a Minimally Defined and Complex Undefined Bacterial Growth Medium.

Nanomaterials (Basel). 2019-11-25

[4]
A two-colour multiplexed lateral flow immunoassay system to differentially detect human malaria species on a single test line.

Malar J. 2019-9-18

[5]
Single and multiple detections of foodborne pathogens by gold nanoparticle assays.

Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2020-1

[6]
Hydrogel based aptasensor for thrombin sensing by Resonance Rayleigh Scattering.

Anal Chim Acta. 2019-6-25

[7]
Carbon-gold hybrid nanoprobes for real-time imaging, photothermal/photodynamic and nanozyme oxidative therapy.

Theranostics. 2019-5-24

[8]
Association between serum albumin level and incidence of end-stage renal disease in patients with Immunoglobulin A nephropathy: A possible role of albumin as an antioxidant agent.

PLoS One. 2018-5-24

[9]
Impact of Particle Size and Polydispersity Index on the Clinical Applications of Lipidic Nanocarrier Systems.

Pharmaceutics. 2018-5-18

[10]
Gold nanoparticle-based enhanced lateral flow immunoassay for detection of Cronobacter sakazakii in powdered infant formula.

J Dairy Sci. 2018-3-1

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索