Fujita Yuka, Ishibuchi Tomoko, Uematsu Akiko, Hayashi Takuya, Obata Fumiaki
Laboratory for Nutritional Biology, RIKEN Center for Biosystems Dynamics Research, Kobe, 650-0047, Hyogo, Japan.
Laboratory of Molecular Cell Biology and Development, Graduate School of Biostudies, Kyoto University, Kyoto, 606-8501, Japan.
Sci Rep. 2025 Aug 23;15(1):31055. doi: 10.1038/s41598-025-16749-0.
Restricting dietary protein intake has metabolic and physiological benefits for animals. Rodent studies have identified the involvement of a hormone, fibroblast growth factor 21 (FGF21), which is upregulated by sensing amino acid scarcity. However, to what extent this mechanism is conserved in primates remains elusive. Using common marmosets, Callithrix jacchus, as a non-human primate model, we develop a protocol for protein restriction and dietary amino acid manipulation. Low protein diet induces a decrease in blood urea nitrogen, altered plasma amino acid profiles, and an increase in plasma FGF21. Supplementation of purified amino acids to the diet suppresses plasma features of protein restriction. Our study provides a dietary intervention technique in marmosets and an insight into the evolutionarily conserved mechanism of FGF21 induction during protein restriction.
限制饮食中的蛋白质摄入量对动物具有代谢和生理益处。啮齿动物研究已确定一种激素——成纤维细胞生长因子21（FGF21）的参与，该激素通过感知氨基酸缺乏而上调。然而，这种机制在灵长类动物中保守的程度仍不清楚。我们使用普通狨猴（Callithrix jacchus）作为非人灵长类动物模型，开发了一种蛋白质限制和饮食氨基酸操纵方案。低蛋白饮食会导致血尿素氮降低、血浆氨基酸谱改变以及血浆FGF21增加。向饮食中补充纯化氨基酸可抑制蛋白质限制的血浆特征。我们的研究提供了一种狨猴饮食干预技术，并深入了解了蛋白质限制期间FGF21诱导的进化保守机制。
