Wahlberg J E, Wrangsjö K, Hietasalo A
Contact Dermatitis. 1985 Oct;13(4):266-9. doi: 10.1111/j.1600-0536.1985.tb02561.x.
100 hospitalized patients with skin disease were patch tested with graded dilutions of nonanoic acid (NON), sodium lauryl sulfate (SLS) and benzalkonium chloride (BENZ). Smooth dose-response curves were obtained. The concentration needed to produce a discernible irritant reaction in 50% of the population (ID50) was found to be lower in cases of irritant than of allergic contact dermatitis; this finding warrants further study. Björnberg's observation that it is not possible to predict the strength of the reaction to one irritant by knowing the strength of the reaction to another was confirmed. Benzalkonium chloride caused a high frequency of pustular and/or bullous reactions with scarring as a sequela. It is suggested that this irritant could be replaced by nonanoic acid in experimental studies of topical irritancy, since the test reactions from nonanoic acid were reproducible, easy to read and left no staining or scarring.
100名住院皮肤病患者用不同浓度稀释的壬酸（NON）、十二烷基硫酸钠（SLS）和苯扎氯铵（BENZ）进行了斑贴试验。获得了平滑的剂量反应曲线。发现在刺激性接触性皮炎病例中，引起50%人群出现可察觉刺激性反应的浓度（ID50）低于过敏性接触性皮炎；这一发现值得进一步研究。证实了比约恩伯格的观察结果，即不可能通过了解对另一种刺激物的反应强度来预测对一种刺激物的反应强度。苯扎氯铵引起脓疱和/或大疱反应的频率很高，会留下疤痕。建议在局部刺激性实验研究中，这种刺激物可用壬酸替代，因为壬酸的试验反应具有可重复性、易于读取且不会留下染色或疤痕。
