Oliva Dreyton, Lardé Hélène, Bouillon Juliette, Whiston Ronan, Peda Andrea, Dowling Patricia, Chapuis Ronan J J
Ross University School of Veterinary Medicine, Basseterre, Saint Kitts and Nevis.
Department of Veterinary Biomedical Sciences, Faculty of Veterinary Medicine, Université de Montréal, Campus de Rimouski, Rimouski, Quebec, Canada.
J Vet Diagn Invest. 2025 Aug 25:10406387251362461. doi: 10.1177/10406387251362461.
To date, the minimum centrifugation times of microhematocrit tubes of blood to generate accurate PCV and total solids (TS) results have not been validated in veterinary medicine. We collected blood samples from 44 donkeys, 43 dogs, 61 sheep, and 40 cattle. We centrifuged microhematocrit tubes for 1, 2, 3, 5, and 15 min in donkeys and dogs, and 1.5, 3, 5, 10, and 15 min in ruminants. We evaluated the agreements between PCV and TS values at each time of centrifugation with the reference values at 15 min using intra-class coefficients of correlation and linear regressions. Finally, we considered the symmetrical distribution of differences between results obtained at each time of centrifugation and the reference values. We found that microhematocrit tubes centrifuged for a minimum of 3 min in donkeys and dogs, 10 min in sheep, and 5 min in cattle gave PCV and TS results in agreement with the values obtained after 15 min of centrifugation. The centrifugation time for cattle was shorter than currently advised. However, because PCV values of all cattle and most donkeys enrolled were within RIs and because no polycythemic animals were included, validation of these times may be warranted.
迄今为止，在兽医学中，尚未验证用于产生准确的红细胞压积（PCV）和总固体（TS）结果的微量血细胞比容管血液的最短离心时间。我们从44头驴、43只狗、61只绵羊和40头牛中采集了血样。我们对驴和狗的微量血细胞比容管分别离心1、2、3、5和15分钟，对反刍动物则分别离心1.5、3、5、10和15分钟。我们使用组内相关系数和线性回归评估每次离心时的PCV和TS值与15分钟时的参考值之间的一致性。最后，我们考虑了每次离心时获得的结果与参考值之间差异的对称分布。我们发现，驴和狗的微量血细胞比容管至少离心3分钟、绵羊离心10分钟、牛离心5分钟时，其PCV和TS结果与离心15分钟后获得的值一致。牛的离心时间比目前建议的要短。然而，由于纳入的所有牛和大多数驴的PCV值都在参考区间内，且未纳入红细胞增多症动物，因此可能需要对这些时间进行验证。
