Zorkóczy Orsolya Krisztina, Turi Orsolya, Wagenhoffer Zsombor, Ózsvári László, Lehotzky Pál, Pádár Zsolt, Zenke Petra
Nutrition and Laboratory Animal Science, Department of Animal Breeding, University of Veterinary Medicine Budapest, H-1078 Budapest, Hungary.
Department of Veterinary Forensics and Economics, University of Veterinary Medicine Budapest, H-1078 Budapest, Hungary.
Animals (Basel). 2023 Jun 23;13(13):2083. doi: 10.3390/ani13132083.
The fallow deer () represents significant game management value globally, and human activities are significantly impacting the species. Besides the positive effects, these activities can threaten its existence, health, and value. The aim of the authors was to develop a tetranucleotide microsatellite panel that could be clearly interpreted and used for genetic testing of fallow deer. Such a panel did not exist until now and could be particularly useful in the field of conservation genetics and forensics. A total of 99 tetrameric microsatellites, originally designed for related deer species, were tested on 20 fallow deer individuals from five Hungarian sampling areas. Original and newly designed primers were used to amplify the microsatellite regions using previously published or optimized PCR protocols. The lengths and sequences of specific amplicons were detected using capillary electrophoresis, and the rate of polymorphism was determined. Altogether, 80 markers provided PCR products of adequate quality and quantity. Among them, 15 markers proved to be polymorphic (2-5 alleles/locus), and 14 tetrameric markers were selected for further analysis. Statistical calculations showed that the selected polymorphic microsatellites can potentially enable key individualization in many areas of wildlife and population genetics, thus protecting the species.
黇鹿（）在全球具有重要的猎物管理价值，而人类活动正对该物种产生重大影响。除了积极影响外，这些活动可能威胁到其生存、健康和价值。作者的目的是开发一种四核苷酸微卫星面板，该面板能够得到清晰解读并用于黇鹿的基因检测。到目前为止，这样的面板并不存在，它在保护遗传学和法医学领域可能会特别有用。总共99个最初为相关鹿种设计的四聚体微卫星，在来自匈牙利五个采样区域的20只黇鹿个体上进行了测试。使用先前发表或优化的PCR方案，用原始和新设计的引物扩增微卫星区域。使用毛细管电泳检测特定扩增子的长度和序列，并确定多态性率。总共有80个标记提供了质量和数量都合适的PCR产物。其中，15个标记被证明具有多态性（每个位点2 - 5个等位基因），并选择了14个四聚体标记进行进一步分析。统计计算表明，所选的多态性微卫星在野生动物和种群遗传学的许多领域有可能实现关键的个体识别，从而保护该物种。
