Marković Milan, Radonjić Dušica, Zorc Minja, Đokić Milena, Marković Božidarka
Biotechnical Faculty, University of Montenegro, Mihaila Lalića 15, 81000 Podgorica, Montenegro.
Biotechnical Faculty, University of Ljubljana, Groblje 3, 1230 Domžale, Slovenia.
Animals (Basel). 2022 Nov 3;12(21):3029. doi: 10.3390/ani12213029.
The Montenegrin sheep population mostly consists of local breeds and their crossbreeds that are very valuable from their genome preservation point of view. The aim of this study was the investigation of the genetic diversity of seven Montenegrin sheep breeds (Jezeropivska-JP, Sora-SOR, Zetska zuja-ZZ, Bardoka-BAR, Sjenička-SJ, Ljaba-Lj, and Piperska zuja-PIP) using 18 microsatellite sets of markers. The genotyping was done for 291 samples from seven populations using the multiplex amplification of sequences with polymerase chain reaction (PCR). The parameters of genetic diversity were estimated using several software tools. In total, 243 alleles were found, with a range of 6 to 25 by locus. The mean observed heterozygosity (Ho), polymorphism information content (PIC), and Fis values (fixation index) per marker were 0.728, 0.781, and -0.007, respectively. The mean number of alleles per breed varied from 4.889 in ZZ to 10.056 in JP. The highest Ho was estimated for JP (0.763) and the lowest for ZZ (0.640). The genetic structure showed close relations between SOR and JP, and both of them with SJ, while ZZ, LJ, and PIP were more distanced. This study provides useful indicators for the development of further in-depth studies and the creation of appropriate conservation programs.
黑山绵羊种群主要由当地品种及其杂交品种组成，从基因组保存的角度来看，这些品种非常有价值。本研究的目的是使用18组微卫星标记来调查七个黑山绵羊品种（耶泽罗皮夫斯卡羊-JP、索拉羊-SOR、泽斯卡祖亚羊-ZZ、巴尔多卡羊-BAR、舍尼奇卡羊-SJ、利亚巴羊-Lj和皮佩尔斯卡祖亚羊-PIP）的遗传多样性。使用聚合酶链反应（PCR）对七个种群的291个样本进行多重序列扩增，从而完成基因分型。使用几种软件工具估计遗传多样性参数。总共发现了243个等位基因，每个位点的等位基因数范围为6至25个。每个标记的平均观察杂合度（Ho）、多态信息含量（PIC）和Fis值（固定指数）分别为0.728、0.781和-0.007。每个品种的平均等位基因数从ZZ的4.889个到JP的10.056个不等。JP的Ho估计值最高（0.763），ZZ的最低（0.640）。遗传结构显示SOR和JP之间以及它们与SJ之间关系密切，而ZZ、Lj和PIP的距离较远。本研究为进一步深入研究的开展和制定适当的保护计划提供了有用的指标。
